谷胱甘肽对Cd的解毒机理研究

Detoxification Mechanism of GSH on Cd

用原子力显微镜研究还原型谷胱甘肽(GSH)对重金属离子Cd的解毒过程,谷胱甘肽和Cd能迅速形成络合物,此络合物有多个活性点,在汞表面能够迅速生长,在开始的1 min的时间内,谷胱甘肽和Cd的络合物是以平均高度为0.574 nm的单分子层吸附在汞的表面,少量形成底面直径达160 nm,高度达46 nm的组装团。随后2 min,端基是活性组装点,整体以向空间垂直方向组装的模式生长。随后侧基的活性显现,侧基的组装和端基的组装同时进行,GSH-Cd不但向垂直方向长高,还向平面方向铺展,形成厚度达18 nm的铺展层和在铺展层上直径为527 nm,高度为80 nm的大分子组装峰。时间达8 min时,各种分子的作用趋于稳定。重金属离子与谷胱甘肽的多个活性基团作用,轻松实现解毒作用。

Using Atomic force microscopy,the experiment studies detoxification process of glutathione（ GSH） on heavy metal ions Cd. Cd complex with Glutathione（ GSH） adsorbed at mercury surface as single molecule in one minute,at which the mean roughness was 0. 574 nm and little part could form mount peak whose diameter could reach 160 nm,and rise 46 nm above the surface. In the following two minutes the end group of glutathione（ GSH） complex with Cd active to form more mounts. Then side group of GSH became active in 5 minutes. The mount became bigger and higher whose diameter could reach 527 nm,and rise 80 nm above the surface,and there was a compacted layer whose thickness was about 18 nm at the foot of the mounts in 8 minutes. Then the system reached a balance,and the biggest mount was formed by about 160 molecule of GSH-Cd. The results show that the stable compound of Cd and GSH will be helpful to the body detoxification.