牡丹红斑病病原鉴定与ITS序列分析

The Identification and Analysis of ITS Sequence on Tree Peony Red Spot

牡丹红斑病是危害牡丹的最重要病害之一。本研究欲通过对病原菌进行鉴定并明确其分类地位。采用形态学方法结合分子标记法对牡丹红斑病病原进行鉴定,利用Mega5软件对枝孢霉属进行系统发育树分析。结果表明:该病致病菌为牡丹枝孢霉,序列与GenBank登录号为GQ395812.1的ITS序列同源性最高,同源性大于99%。聚类分析发现,枝孢霉属可分为7个聚类组,样品菌株与牡丹枝孢霉为一类。分子鉴定与形态学鉴定结果一致,该研究为牡丹红斑病的快速诊断与防治提供了依据,并为枝孢属的系统发育和分类鉴定提供了参考。

The red spot was one of the most important diseases which harms peony tree. The aim of the research was to clarify its taxonomic status by identifying the pathogen of tree peony leaf spot. In this study, the pathogen was identified based on morphology and molecular identification, the ITS phylogenetic trees of CIadosporium spp. was generated with the soft of Mega 5. The result suggested that the pathogen of tree peony leaf spot was CIadosporium paeoniae, and its sequences had the highest homology with GQ395812.1 in GenBank, whose homology was greater than 99%. Cluster analysis suggested that the Cladosporium spp. could be divided into 7 groups. Samples strains and CIadosporium paeoniae belonged to a class. The molecular identification and morphology identification researched the same results. This paper provided the reference for the rapid diagnosis and prevention of tree peony red spot disease, and the information for phylogenetic and taxonomic identification of Cladosporium spp.