清火解毒法对实验小鼠的镇痛抗炎作用研究

Research on the Analgesic and Anti-inflammatory Effect on Mice by Clearing Fire and Detoxification Method

目的:从药理角度探讨清火解毒法的镇痛抗炎作用。方法:乙酸刺激至小鼠疼痛扭体实验、小鼠热板实验,观察中药对小鼠疼痛的抑制作用;二甲苯致小鼠耳肿胀法,比较各组用药后小鼠耳壳肿胀度;醋酸诱发小鼠毛细血管通透性增加,观察中药对小鼠腹腔毛细血管通透性的影响。结果:可明显减少乙酸所致小鼠的扭体反应次数(P<0.01)。明显提高小鼠的痛阈值(P<0.01)。能减轻小鼠耳壳肿胀度及肿胀率(P<0.05,P<0.01);降低由醋酸引起的小鼠毛细血管通透性的增加(P<0.05)。结论:清热解毒法具有一定的镇痛抗炎作用。

Objective： From the pharmacological point of view,to explore the analgesic and anti-inflammatory effect of clearing fire and detoxification therapy.Method：Acetic acid stimulation caused the mice to pain writhing and hot plate experiment,and observe the effect on the inhibition of pain in mice by taditional Chinese medicine.Xylen induced ear e-dema in mice, comparing the auricular swelling of the mice in each group after treatment.Acetic acid induced the addi-tion of capillary permeability in mice,and observe the impact of capillary permeability in mice by taditional Chinese med-icine.Results：Reduce the number of writhing response in mice caused by acetic acid significantly（P〈0.01）.Increase the pain threshold in mice significantly（P〈0.01）.Reduce the auricular swelling and swelling rate in mice（P〈0.05, P〈0.01）;Reduce the addition of capillary permeability in mice caused by the acetic acid （P〈0.05）.Conclusion：Clearing fire and detoxification therapy has some analgesic and anti-inflammatory effects.