阿魏酸钠对DBI大鼠耳蜗NF-κB表达的影响

EFFECTS OF SODIUM FERULATE ON THE EXPRESSION OF NF-κB IN COCHLEAR WITH DIFFUSE TRAUMATIC BRAIN INJURY MODEL OF RATS

目的探讨阿魏酸钠对弥漫性脑外伤后耳蜗核转录因子-κB表达的影响。方法将SD大鼠随机分为三组:正常对照组、脑外伤组及阿魏酸钠治疗组,以蛋白印迹及免疫组织化学法观察弥漫性脑外伤后NF-κB表达的变化及阿魏酸钠对NF-κB表达的影响。结果脑外伤后24,72,168h的耳蜗组织内,NF-κB表达较正常对照组均明显增高,而阿魏酸钠治疗组,NF-κB表达较损伤组均明显下降(P<0.01)。结论脑外伤后耳蜗内NF-κB表达显著增高,阿魏酸钠对耳蜗的保护作用可能与NF-κB表达降低有关。

Objective To investigate the effects of sodium ferulate on the exporession of nuclear factor-κ-gene binding（NF-κB）in cochlear with diffuse traumatic brain injury model of rats.Methods Diffuse traumatic brain injury model of rats were established and randomly divided into three groups,including normal control group,diffuse traumatic brain injury model group and sodium ferulate treatment group.Then Western blot and immunohistochemical staining method were used to evaluate the expression of nuclear facthor-κ-gene binding in cochlear and the effects of sodium ferulate.Results The expression of NF-κB in cochlear tissues were significantly increased after brain injury of twenty four hours,seventy two hours and one hundred and sixty eight hours than normal control group.Whereas the expression of NF-κB in sodium ferulate group was significantly decreased than model group（P〈0.01）.Conculsion The expression of NF-κB in cochlear tissues is significantly increased in brain injury model,the protective effect of sodium ferulate on cochlear in diffuse traumatic brain injury model of rats may be related to the reduction of the NF-κB.