贵阳地区汉族人群 HPA-1～5基因多态性与2型糖尿病合并颈动脉粥样硬化的关系

Analysis of association between genetic polymorphism of human platelet antigens HPA-1-5 and type 2 diabetes combined with carotid atherosclerosis of Han people in Guiyang

目的：研究贵阳地区汉族人群中人类血小板抗原（HPA）1～5基因多态性与2型糖尿病合并颈动脉粥样硬化的相关性。方法2型糖尿病患者99例（糖尿病组），按有无颈动脉粥样硬化分为CA （＋）组（ n＝56）和CA （-）组（ n＝43）两个亚组，同期健康体检者100例作为正常组，采用酚-氯仿法提取基因组DNA，序列特异性引物-多聚酶链反应（SSP-PCR）技术扩增目的基因，进行HPA基因分型。结果正常组、CA（＋）组、CA（-）组三组间的HPA-1、2、3、5等位基因频率及基因型比较，差异均有统计学意义（ P均＜0．05）；正常组与糖尿病组的HPA-1、2、3等位基因频率及基因型比较，差异均有统计学意义（P均＜0．05）；CA（＋）组与CA（-）组的HPA-5等位基因频率及基因型比较差异有统计学意义（P＜0．05）。经Logistic回归分析，年龄、糖尿病病程、高血压、吸烟、超重、血脂异常、HPA-5基因多态性为2型糖尿病患者颈动脉粥样硬化发生的危险因素（ P均＜0．05）。结论 HPA-1、2、3基因多态性与贵阳地区2型糖尿病的发生可能存在相关性，HPA-5基因多态性可能是2型糖尿病合并颈动脉粥样硬化发生的危险因素。

Objective To study the association between genetic polymorphism of human platelet antigens HPA -1-5 and type 2 diabetes combined with carotid atherosclerosis （ CA） of Han people in Guiyang .Methods 99 patients with type 2 diabetes （diabetes group） and 100 healthy controls （normal group） were enrolled in this case-control study.The di-abetes group was devided into two groups , which was CA（ ＋） （combined with CA） and CA（ -） （non-CA）.Phenol-chloroform method was used to extract the genomic DNA and sequence-specific primers-polymerase chain reaction （ SSP-PCR） technique was applied for amplification of the target gene and genotyping .Results The allele frequency and geno-type of HPA-1, 2, 3, 5 showed significant differences among the normal group , CA（＋） group and CA（-） group （all P〈0.05）.The allele frequency and genotype of HPA-1, 2, 3 showed significant differences between the normal group and the diabetes group （all P〈0.05）.The allele frequency and genotype of HPA-5 showed significant differences between CA （＋） and CA（-） （P〈0.05）.Logistic regression analysis showed that age , duration of diabetes, hypertension, smok-ing, overweight, dyslipidemia, genetic polymorphism of HPA-5 were risk factors of CA in patients with type 2 diabetes. Conclusions There is a possible correlation between genetic polymorphism of human platelet antigens HPA -1, 2, 3 and occurrence of type 2 diabetes.Genetic polymorphism of HPA-5 may be the risk factor of type 2 diabetes with CA.