摘要
为明确生防菌NJQG-3A1菌株在香蕉植株根、球茎、假茎、叶和根际土壤中的定殖情况和对香蕉枯萎病的生防作用,为香蕉枯萎病的生防治理提供理论依据,采用抗生素标记法测定菌株NJQG-3A1的定殖能力,以盆栽试验测定其对香蕉枯萎病的防控效果。定殖测定结果表明:以重铬酸钾(100μg/mL)-氨苄青霉素(100μg/mL)标记的NJQG-3A1菌株,可在香蕉根际土壤和植株的根、球茎、假茎中定殖,定殖量表现为:根际土壤〉根〉球茎〉假茎〉叶;在土壤中的定殖量可达1×10^5cfu/g;在香蕉植株内定殖量为1×10^2~1×10^3cfu/g。盆栽试验结果表明:经NJQG-3A1菌株的发酵液处理后,香蕉植株的株高、茎围、鲜重比对照(接种病原菌,只施用清水)分别提高了99.22%,44.60%,218.67%,与对照差异显著(P〉0.05);同时,菌株NJQG-3A1对香蕉枯萎病的防控效果显著,叶部与根茎部的病情指数分别为15、10;病情防效为81.82%,88.98%。NJQG-3A1菌株可以在香蕉植株和根际土壤中定殖,且该菌株的发酵液对香蕉枯萎病具有良好的防控效果,对香蕉植株的生长具有促进作用。
The colonization capacity of NJQG- 3A1 strain in banana plants and the rhizosphere was investigated, and the control efficacy of NJQG-3A1 against banana fusarium wilt was also studied, which will provide a theoretical basis for ecological control of the disease. The colonization trends of NJQG- 3A1 in banana plants and the rhizosphere was examined with the method of antibiotic-resistance. Furthermore, the effect of NJQG-3A1 against banana fusarium wilt was investigated by using pot experiment method. Results showed that NJQG- 3A1, tagged by the resistance of‘Ampicllin(100 μg/mL)- Potassium dichromate(100 μg/mL)', could colonized in roots, corms and pesudostems tissue of banana and rhizosphere soil stably.The population of NJQG-3A1 in different part was: rhizosphere soil〉roots〉corms〉pesudostems〉 leaves. It was also found that the population colonied in the rhizosphere and the banana plant was 1×10^5cfμ/g, 1×10^2to1×10^3cfμ/g respectively. In addition, the pot experiment result showed that the banana plant height, steam circumference, fresh weight were increased by 99.22%, 44.60%, 218.67% respectively, significantly different from the control(P〈0.05), when treated with the strain NJQG-3A1 fermentation liquid strain NJQG-3A1 also showed obvious biocontrol effect on banana banana fusarium wilt, the disease indexes of leaves and root-steam were 15, 10 respectively, and the control efficacy were 81.82%, 88.98%, respectively. NJQG- 3A1 could colonize in the banana plant and the rhizosphere. The strain NJQG-3A1 fermentation liquid showed strong disease biocontrol effect on banana fusarium wilt, as well as promotion effect on banana plant growth.
出处
《中国农学通报》
CSCD
2014年第28期280-285,共6页
Chinese Agricultural Science Bulletin
基金
中国热带农业科学院院本级基本科研业务费专项"不同硝铵比对香蕉根际微生物群落多样性的影响"(1630052014011)
现代农业产业技术体系建设专项"国家香蕉产业技术体系栽培技术与耕作岗位"(CARS-32-06)
关键词
香蕉枯萎病
内生放线菌
定殖
防控效果
banana fusarium wilt
Endophytic actinomycete
colonization
control effect