摘要
为调查桑花叶萎缩类病毒的多态性,通过RT-PCR技术分离了安康地区桑花叶型萎缩类病毒,并对其基因组进行了克隆测序和序列分析。结果获得4个MMDVd变体,核苷酸长度为356 nt或357 nt,GC含量均约51%,Mfold网站预测二级结构两端为分枝状结构,其中约有58%的碱基配对。
To detect the polymorphism of mulberry mosaic dwarf viroid, the sequence of mulberry mosaic dwarf viroid isolates in Ankang were investigated by RT-PCR, cloning and sequence analysis. The results showed that 4 variants of MMDVd were obtained, and the length was from 356 nt to 357 nt. The GC content was about 51% for all. There were about 58% pairs in MMDVd secondary structure predicted in Mfold web, and the two end of it were both branched.
出处
《中国农学通报》
CSCD
2014年第28期306-309,共4页
Chinese Agricultural Science Bulletin
基金
陕西省青年科技新星项目"桑树类病毒在非自然宿主中的复制潜能研究"(2013KJXX-96)
安康学院教育教学改革研究项目"基于研究的生物科学类本科毕业设计模式的研究与实践"(Jg06105)
大学生创新创业训练计划项目"桑花叶萎缩类病毒基因组序列与二级结构的多态性研究"(2013sxjy006)
关键词
桑花叶萎缩类病毒
检测
序列多态性
二级结构
mulberry mosaic dwarf viroid
detection
sequence polymorphism
secondary structure
