药用牡丹种子质量检验方法的研究

Seed quality test methods of Paeonia suffruticosa

该文参考国际植物种子检验规程和农作物种子检验规程的方法,测定了不同产地药用牡丹种子质量,探索药用牡丹种子质量检验方法,为制定药用牡丹种子检验规程和质量标准提供依据。初步建立了药用牡丹种子质量检验方法,药用牡丹种子净度分析送检样本最少7 000 g,试验本最少700 g;真实性鉴定采用种子外观形态比较;千粒重测定采用百粒法;含水量测定采用低恒温烘干法10 h;室温清水浸泡24 h,黑暗条件下昼夜变温层积（20℃,12 h和25℃,12 h）60 d后,用300 mg·L^-1GA3浸泡种子24 h,以砂中作为发芽床,在15℃光照条件下萌发,发芽首末次计数时间为12~60 d;生活力测定采用TTC染色法。

In order to optimize the testing methods for Paeonia suffruticosa seed quality, and provide basis for establishing seed testing rules and seed quality standard of P. suffruticosa. The seed quality of P. suffruticosa from different producing areas was measured based on the related seed testing regulations. The seed testing methods for quality items of P. suffruticosa was established preliminarily. The samples weight of P. suffruticosa was at least 7 000 g for purity analysis and was at least 700 g for test. The phenotypic observation and size measurement were used for authenticity testing. The 1 000-seed weight was determined by 100-seed method, and the water content was carried out by low temperature drying method （10 hours）. After soaking in distilled water for 24 h, the seeds was treated with different temperature stratifications of day and night （25 ℃/20 ℃, day/night） in the dark for 60 d. After soaking in the liquor of GA3 300 mg · L^-1 for 24 h, the P. suffruticos seeds were cultured in wet sand at 15 ℃ for 12-60 days for germination testing. Seed viability was tested by TTC method.