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白癜风皮肤组织角质形成细胞释放警报素分子的研究 被引量:4

The study of release of alarmin HMGB1 in keratinocytes from skin tissues in vitiligo
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摘要 目的:探讨人永生化角质形成细胞(HaCaT)和人白癜风组织表达与释放高迁移率蛋白B1(HMGB1)。方法:HaCaT分别经紫外线照射、加凋亡诱导剂、细胞因子刺激、反复冻融处理,细胞培养上清经浓缩后,Western-blot观察24h后HMGB1的释放。免疫荧光染色检测白癜风患者和对照组的活检皮肤组织HMGB1和激活的半胱氨酸蛋白酶3(cleaved caspase-3)的表达。结果:HaCaT经各种处理后,培养上清中均能检测出HMGB1,对照组则不能。白癜风病人角质层检测到cleaved caspase-3,对照组病人未检测到;对照组HMGB1表达在角质形成细胞核内,白癜风组织HMGB1从细胞核转移至细胞浆。结论:HMGB1能够由角质形成细胞在凋亡和坏死时被动释放或者在病理状态下(白癜风)主动分泌。 Objective :To investigate the release of HMGB1 by HaCaT cell and keratinocytes of tissue from vitiligo patients .Methods :HaCaT cells were treated with UV light or with apoptosis drugs or with cytokines and freeze-thaw process . The supernatant was condensed and subjected to SDS-PAGE to detect the release of HMGB1 .Measure the HMGB1 and cleaved caspase-3 expression in skin biopsies of normal control subjects and viti-ligo patients by immunofluorescence .Results :HMGB1 was detected in the supernatant of HaCaT after treated and control supernatant was not detected .Cleaved caspase-3 was found in the stratum corneum of vitiligo patients and control patient was not found .HMGB1 was expressed in the nucleus of keratinocytes of control patients and trans-ferred from nucleus to cytoplasm in vitiligo patients .Conclusion:HMGB1 can be passively released by apoptosis and necrosis keratinocytes or actively released in vitiligo .
作者 牟宽厚 李盼
出处 《陕西医学杂志》 CAS 2014年第10期1275-1277,共3页 Shaanxi Medical Journal
基金 国家自然科学基金资助项目(81171504 81202352)
关键词 白癜风 皮肤系统 高迁移率族蛋白质类 角质形成细胞 Vitiligo Integumentary system High mobility group proteins @Keratinocytes
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