血管紧张素转换酶基因多态性与2型糖尿病肾脏疾病的相关性研究

Association of angiotensin converting enzyme gene polymorphism with type 2 diabetic kidney disease

目的:探讨血管紧张素转换酶(ACE)基因多态性与2型糖尿病肾脏疾病的关系。方法:采用聚合酶链反应(PCR)方法检测28例糖尿病肾病患者(DN组)、30例非糖尿病性肾脏疾病患者(MD+NDRD组)、18例DN合并NDRD患者(DN+NDRD组)和30例健康体检者(正常对照组)的ACE I/D基因多态性。结果:1DN组和DN+NDRD组ACE-DD基因型频率明显高于DM+NDRD组和正常对照组(P<0.05),D等位基因频率明显高于DM+NDRD组(P<0.05)和正常对照组(P<0.01),DM+NDRD组和正常对照组比较以及DN组和DN+NDRD组比较ACE-DD基因型和D等位基因频率均无明显差异(P>0.05)。2ACE-DD基因型为2型糖尿病肾脏疾病患者eGFR下降的危险因素。结论:1ACE-DD基因型以及D等位基因与2型糖尿病患者DN和DN+NDRD的发生有关,与DM+NDRD的发生无关。2ACE-DD基因型是2型糖尿病肾脏疾病患者肾功能减退的易感因素。

Objective：To investigate the relationships of gene polymorphisms of angiotensin converting en-zyme （ACE） with type 2 diabetic kidney disease .Methods ：The allele frequency and the genotype distribution of ACE I/D gene polymorphism were studied in 28 type 2 diabetes mellitus patients with diabetic nephropathy （DN） , 30 patients with non-diabetic renal disease （DM＋NDRD） ,18 patients with both DN and NDRD （DN＋NDRD） and 30 healthy controls by means of polymerase chain reaction （PCR） .Results ：① The frequency of ACE-DD genotype was significantly higher in DN and DN＋NDRD groups than that in DM ＋NDRD and healthy control groups （P〈0 . 05） .The frequency of ACE D allele was significantly higher in DN and DN ＋NDRD groups that than in DM ＋NDRD （P〈0 .05） and healthy control （P〈0 .01） groups .No significantly differences were found in ACE-DD genotype or ACE D allele among DN and DN＋NDRD groups ,and among DM＋NDRD and healthy control groups .②ACE-DD genotype was the risk of low estimated glomerular filtration rate （eGFR） .Conclusion ：① ACE-DD genotype and ACE D allele are significantly associated with DN and DN ＋NDRD in type 2 diabetes mellitus patients .There may be no association between ACE-DD genotype or ACE D allele and the initiation of DM ＋ NDRD .②ACE-DD genotype may predispose the increased risk of eGFR degression in patients with type 2 diabetic kidney disease .