依达拉奉对吸入性肺损伤大鼠肺组织的保护作用

Protective effect of Edaravone on inhalation lung injury caused by black gunpowder smoke in rats

目的探讨依达拉奉对黑火药烟雾所致吸入性肺损伤大鼠的保护作用。方法将24只雄性Wistar大鼠随机分为正常对照组(C组)、吸入性肺损伤组(S组)和依达拉奉组(E组),每组8只。除正常对照组外,其余各组大鼠使用自制发烟装置构建吸入性肺损伤模型。造模成功后依达拉奉组按9 mg/kg腹腔注射依达拉奉注射液;正常对照组、吸入性肺损伤组腹腔注射0.9%氯化钠注射液12 ml/kg。于第7天从大鼠腹主动脉取血,检测各组大鼠动脉血气;肺湿/干重比(lung wet-todry weight ratio,W/D);留取各组大鼠腹主动脉血液样本,离心取血清,待检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)含量。留取肺组织,制备肺组织匀浆后测定髓过氧化物酶(myeloperoxidase,MPO)、丙二醛(malondialdehyde,MDA)含量。取部分右肺组织,经4%甲醛溶液固定后做病理苏木精-伊红(HE)染色切片,光镜观察。结果与S组相比,E组大鼠动脉中Pa O2水平升高(P<0.01),大鼠血清中IL-6、TNF-α水平降低并降低肺组织中MPO活性及MDA含量(P<0.01)。光镜下观察,E组肺组织较S组肺组织水肿减轻,炎性细胞浸润减少。结论依达拉奉可能通过抑制自由基的产生及减少部分炎性介质的产生和释放,减轻烟雾吸入性肺损伤大鼠体内炎性反应,对肺组织起到一定的保护作用。

Objective To explore the protective effect of Edaravone on inhalation lung injury caused by black gunpowder smoke in rats.Methods Twenty four male Wistar rats were randomly divided into three groups （n=8）：normal control group （C）,inhalation lung injury group （S）,Edaravone group （E）.The rat model of inhalation lung injury was reproduced by a self-made smoke generator.Rats in E group were given 9 mg/kg of Edaravone by intraperitoneal injection.And at the same time the control group （C） and inhalation group （S） were injected normal saline （12 ml/kg）.The animals were sacrificed seven days later,and the arterial blood gas values,lung wet-to-dry weight ratio （W/D） were tested.The blood samples were centrifugalized and the content of tumor necrosis factor-α （TNF-α）,interleukin-6 （IL-6） in serum were determined.The content of myeloperoxidase （MPO） activity and malondialdehyde （MDA） in lung tissue were detected.And the right lung tissue which was fixed by 4％ of formaldehyde solution was observed by HE.Results The level of PaO2 in group E were higher than in group S （P ＜ 0.01）,however,TNF-α,IL-6 in serum,MPO activity in lung tissue and MDA content in group E were lower than in group S （P ＜ 0.01）.Histopathological observation showed less inflammatory exudates and infiltrations in group E than in group S.Conclusion Edaravone shows a protective effect on inhalation lung injury caused by black gunpowder smoke in rats by inhibiting the production of free radical and the release of inflammatory mediators.