颈型颈椎病家兔模型的Ca2＋-ATP酶及SOD、MDA含量变化

The Content Changes of Ca2＋-ATP Enzyme and SOD, MDA of Rabbit Model with Neck Type Cervical Spondylosis

目的：探讨处于强迫体位时骨骼肌损伤的机理，为各种非手术疗法（中药、针灸、理疗、推拿等）防治颈椎病和该病的术后康复医疗提供理论依据。方法：建立颈型颈椎病家兔模型，应用颈部肌肉的变性理论，对处于强迫体位不同时期的家兔血清肌肉超氧化物歧化酶（SOD）和丙二醛（MDA）、Ca2＋-ATP酶变化情况进行研究。结果：与空白组相比，造模组家兔颈椎后部肌肉组织中Ca2＋-ATP酶活力及SOD含量显著下降（P〈0.05），MDA含量增高（P〈0.05）；与造模组I相比，造模组II Ca2＋-ATP酶活力及SOD含量显著升高（P〈0.05），MDA含量显著降低（P〈0.05）。结论：造模后，家兔颈椎后部肌肉组织细胞的清除自由基功能受抑制，脂质过氧化作用增强。

Objective：To investigate the mechanism of skeletal muscle injury on forced posture, providing theoretical basis for various non-operative therapy （Chinese medicine, acupuncture, physiotherapy, massage） preventing cervical spondylosis and its postoperative rehabilitation. Methods：Es-tablishing the rabbit model of neck type cervical spondylosis, applied with degeneration theory of neck muscle, rabbit serum muscle superoxide dis-mutase （SOD） and malonaldehyde （MDA） and Ca2＋-ATP enzyme of forced position in different periods were studied. Results：Compared with the blank group, Ca2＋-ATP enzymatic activity and SOD content of posterior cervical muscle tissue of model group significantly decreased （P〈0.05）,and MDA content increased （P〈0.05）;compared with model group I, Ca2＋-ATP enzymatic activity and SOD content of model group II increased signifi-cantly （P〈0.05）, and MDA content decreased significantly （P〈0.05）. Conclusion：The function of posterior cervical muscle tissue cells scavenging free radicals is inhibited, while lipid peroxidation is enhanced after modeling.