儿童哮喘与幽门螺杆菌感染的相关性研究

A study on relationship between childhood asthma and infection helicobacter pylori

目的通过对比分析哮喘患儿与健康儿童幽门螺旋杆菌（Hp）14C感染率以及血浆中白细胞介素-1b（IL-1b）含量的关系,分析探究Hp感染对儿童哮喘的作用机理,找出更佳防治儿童哮喘的对策方法。方法采用分层抽样法随机抽取2010年8月~2012年8月在我院住院治疗的50例哮喘患儿（哮喘组）、50例非哮喘Hp感染患儿（Hp感染正常组）、50例哮喘且Hp感染患儿（Hp感染＋哮喘组）和50例健康儿童（正常组）为受试对象。利用14C尿素呼气检测仪检测Hp感染阳性率,ELISA试剂盒检测血浆中白细胞介素-1b（IL-1b）含量。结果 1哮喘儿童的14C尿素呼气检测阳性率为34.0%（哮喘组）和50.0%（Hp感染＋哮喘组）,明显大于健康对照儿童的4.0%（P〈0.05）,且哮喘和Hp感染正线性相关（R=0.30,P〈0.05）;2Hp菌株感染后的儿童14C尿素呼气检测阳性率明显高于未感染儿童（P〈0.05）,且哮喘和Hp阳性感染概率呈现正线性关系（R=0.47,P〈0.05）。3四组患儿IL-1b都满足正态分布,其中哮喘儿童的IL-1b浓度为（3.68±0.12）ng/m L,低于健康对照儿童的（0.61±0.04）ng/m L,组间对比具有统计学意义（P〈0.05）;Hp感染＋哮喘组儿童IL-1b浓度为（4.77±0.09）ng/m L,明显高于哮喘组儿童的（3.68±0.12）ng/m L,组间对比差异明显（P〈0.05）。结论哮喘儿童与无哮喘儿童幽门螺旋杆菌（Hp）感染率和血浆中白细胞介素-1b（IL-1b）含量有显著差异,这为哮喘患儿诊断提供了监测依据,在儿童哮喘疾病的治疗过程中有较大的应用价值。

Objective By analyzing children with asthma and healthy children with helicobacter pylori （Hp） infection rate of 14C and the serum levels of interleukin -lb （IL-lb） analysis of the content to explore the relationship between the mechanism of asthma in children infected with Hp and to find a better method for prevention of asthma in children. Methods Stratified sampling randomly from August 2010 to August 2012 in our hospital treatment of 50 cases of chil- dren with asthma （asthma group） and 50 cases of children with asthma Hp infection （Hp infection normal group） and 50 cases of children with asthma and Hp infection （lip infection＋asthma group） and 50 cases health children （normal group） as the subjects. Used 14C urea breath detector to detect Hp infection positive rate, ELISA kits in plasma interleukin lb （IL-1 b） content. Results （1） 1% urea breath test positive rate of asthma children was 34.0% （asthma group） and 50.0% （Hp infection＋asthma group）, which was significantly greater than healthy controls 4.0% of the children, （P〈0.05）, and asthma and Hp infection was linear correlation （R=0.30, P〈0.05）; （2） Hp strains infection after 1% urea breath test positive rate was significantly higher than those uninfected children （P〈0.05）, and asthma and Hp positive probability of infection showed positive linear relationship （R=0.47, P〈0.05）. （3） The four groups of children with IL-lb could meet the normal distribution and the concentration of IL-lb for asthma children was （3.68±0.12） ng/mL, lower than that in the healthy control children （0.61±0.04） ng/mL. Comparison between groups had statistical significance （P〈0.05）; Hp infection ＋concentration of IL-lb for children asthma group was （4.77±0.09） ng/mL, significantly higher than that of asthma group children （3.68±0.12） ng/mL. The comparison between the two groups had obvious difference （P〈0.05）. Conclusion Helicobacter pylori （Hp） infection and the serum levels of interleukin -lb （IL-lb） in children with asthma and with- out asthma have significantly different content, which provides the basis for the diagnosis of children with asthma mon- itoring, and has great application value in the treatment of disease of asthmatic children.