转3α-HSD基因水稻对土壤根际微生物影响及胆固醇降解菌的筛选鉴定

Effect of 3α-HSD Transgenic Rice on the Microorganism in the Soil and Isolation and Identification of Cholesterol-Degrading Strains in the Soil

通过对转3α-HSD基因水稻各个时期根际微生物的数量的研究,探究转基因水稻对土壤微生物的影响,并且从种植转基因水稻的土壤中分离纯化出两株具有较强降解胆固醇的菌株。结果表明,转基因水稻的根际微生物在数量上大于常规水稻。通过从土壤中富集分离纯化分离出来的2个菌株在30℃,180 r/min的条件下培养4天后对0.1 mg/m L胆固醇的胆固醇降解率分别达到38.75%和64.64%,通过经形态特征以及16S rDNA序列分析,初步鉴定为肠杆菌科克氏杆菌(Klebsiella pneumoniae)。

In this paper, 3α-HSD transgenic rice rhizosphere microorganism of the soil were cultured and counted to reveal the effects of rhizosphere microbial secretions on the number of microorganisms at different times. And 2 strains that utilized cholesterol as sole carbon and energy sources were isolated from the soil by the enrichment culture method. The results showed that, the quantities of 3α-HSD transgenic rice rhizosphere microorganism were greater than that of conventional rice. Two strains were selected for degradation of cholesterol. In the culture conditions of 30℃ and 180 r/min, the degradation rate to cholesterol of 2 strains were 38.75% and 64.64%, with 0.1 mg/mL concentrations within four days. 2 strains were identified to be Klebsiellapneumoniae by morphological characteristics and the similarity analysis of the 16S rDNA sequence.