大豆基因组WRKY31同源基因的生物信息学分析

Bioinformatics Analysis of WRKY31 Homologs in Soybean Genome

WRKY转录因子是一类参与多种生物和非生物胁迫的诱导性转录调控因子,在植物进化上高度保守。通过克隆大豆WRKY31基因全长和生物信息学分析,发现WRKY31在大豆基因组中存在3个同源基因,分别位于第6、12和13号染色体上;WRKY31蛋白含有1个WRKY结构域（343-419 aa）和C2H2型锌指结构,属于第二类WRKY基因。RT-PCR分析表明,GmWRKY31在大豆的叶、花、豆荚、根、根瘤中的组织内均有表达,且在花中表达量最高,豆荚中最低。通过MEGA 5.0软件分析,大豆和芸豆WRKY蛋白亲缘关系更近,位于同一分支上,而它的2个拷贝Glyma06g46420.2和Glyma12g10350.1位于另一个分支上,亲缘关系较近。蛋白结构及功能预测表明WRKY31是一类转录因子,参与胁迫反应;系统进化分析表明其基因在进化过程中功能上发生了重大分化。

The gene family of WRKY is transcription factor that can be induced and involved in the response to biotic and abiotic stresses,and is conserved evolutionarily. In this research,the full-length cDNA of GmWRKY31 was cloned and bioinformatics analysis was used to predict the potential function of GmWRKY31. Phylogenetic analysis of GmWRKY31 and its homologs with different plant species were conducted by the software MEGA 5. 0. The results showed that three copies of WRKY31 exited in the soybean genome,located on the chromosome 6,12 and 13. GmWRKY31 belongs to the type II WRKY transcription factors,with a WRKY domain and a C2H2-type zinc finger. Real-time RT-PCR analysis showed that GmWRKY31 was expressed in soybean leaf,flower,pod,root,nodule,and the flower had the highest expression,the lowest expression was in the pod. The result of the phylogenetic tree indicted that WRKY31 from Glycine max and Phaseolus vulgaris were the nearest in genetic relationship. While Glyma06g46420. 2 and Glyma12g10350. 1（the other two copies of WRKY31 of Glycine max） had closer relationship. The result of protein structure and function prediction showed that GmWRKY31 was a transcription factor being involved in the stress response of plant,however,the phylogenetic tree indicted that significant difference of the WRKY genes happened in evolution of soybean.