乳腺癌组织中Tuberin和磷酸化Girdin蛋白的表达及临床意义

Expressions of Tuberin and phosphorylated Girdin in breast cancer and their clinical significances

目的探讨乳腺癌组织中Tuberin和磷酸化Girdin(p-Girdin)蛋白的表达及其与乳腺癌临床病理特征和分子分型的关系。方法应用免疫组化Envision法检测102例乳腺非特殊型浸润性导管癌、52例乳腺导管原位癌、31例乳腺纤维腺瘤组织中Tuberin及p-Girdin蛋白的表达情况。结果 Tuberin在乳腺非特殊型浸润性导管癌、乳腺导管原位癌、乳腺纤维腺瘤组织中的阳性表达率分别为33.3%、59.6%和77.4%,差异有统计学意义(P<0.05)。Tuberin与乳腺非特殊型浸润性导管癌患者年龄、病理学分期、组织学分级、肿瘤大小、淋巴结转移、雌激素受体(ER)、孕激素受体(PR)、人表皮生长因子受体(HER-2)无关(P>0.05)。Tuberin在管腔A型、管腔B型、HER-2(+)型和三阴性乳腺非特殊浸润性导管癌中的阳性表达率分别为34.0%、40.0%、31.3%和21.4%,差异无统计学意义(P>0.05)。p-Girdin在乳腺非特殊型浸润性导管癌、乳腺导管原位癌和乳腺纤维腺瘤组织中的阳性表达率分别为64.7%、38.4%和22.6%,差异有统计学意义(P<0.05)。p-Girdin与乳腺非特殊型浸润性导管癌的病理学分期、淋巴结转移数及HER-2状态呈正相关(P<0.05),与患者年龄、组织学分级、肿瘤大小、ER、PR无相关性(P>0.05)。p-Girdin在乳腺非特殊浸润性导管癌的四种分子分型中的阳性表达率分别为57.4%、92.0%、62.5%和42.9%,差异有统计学意义(P>0.05)。Tuberin与p-Girdin在乳腺非特殊浸润性导管癌中的表达无关(P>0.05)。结论乳腺癌组织中Tuberin的表达明显降低,可能具有抑制乳腺癌增殖、侵袭、转移的作用,而p-Girdin的表达可能与乳腺癌的恶性进展密切相关。

Objective To detect the expressions of Tuberin and phosphorylated Girdin（ p-Girdin）in breast cancer and its association with pathological characteristics and molecular subtypes of breast cancer.Methods Immunohistochemical Envision chemical method was used to investigate the expression of Tuberin and phosphorylated Girdin（ p-Girdin） in 102 cases of non-special type invasive carcinoma（ IDCNOS） of breast,52 cases of ductal carcinoma in situ（ DCIS）,and 31 cases of breast fibroad-noma.Results The expression of Tuberin protein positive expresstion rate was 33.3%,59.6%,77.4%,respectively.There was significant difference among IDC-NOS,DCIS and breast fibroadnoma（ P〉 0.05）.The expression rate of Tuberin was not related to age,pathologic stage,histological grade,tumor size,lymph node metastasis,estrogen receptor（ ER）,progesterone receptor（ PR） of the patients with IDC-NOS（ P〈 0.05）.The expression of Tuberin in luminal A,luminal B,human epidermal growth factor receptor 2（ HER-2）（ ＋）type and triple negative were 34.0%,40.0%,31.3%,21.4%,respectively.There was no statistically significant difference（ P〈 0.05）.The expression of p-Girdin protein positive expresstion rate were 64.7%,38.4%,22.6%,respectively.There was statistically significant difference among IDC-NOS,DCIS and breast fibroadnoma（ P〈 0.05）.The expression of p-Girdin was positively associated with pathologic stage,lymph node metastasis and HER-2（ P〈 0.05）.But no significant association was identified between p-Girdin expresstion and age of patients,histological grade,tumor size,ER,PR（ P〈 0.05）.The expression of p-Girdin in luminal A,luminal B,HER-2（ ＋） type and triple negative were 57.4%,92.0%,62.5%,42.9%,the difference was statistically significant（ P〈 0.05）.The expression of Tuberin and p-Girdin among the IDC-NOS patient has no relevance（ P〉 0.05）.Conclusion The expression of Tuberin in breast cancer tissues was reduced significantly,and may have inhibition on breast cancer tissues＇ s proliferation,invasion,metastasis.The expression of phosphorylated Girdin may have relationship with deterioration of breast cancer.