海洋链霉菌L211次生代谢产物分离和结构鉴定

Isolation and identification of secondary metabolites produced by a marine-derived Streptomyces sp. L211

利用硅胶柱、凝胶柱及制备薄层色谱等色谱方法对海洋链霉菌Streptomyces sp.L211发酵液的乙酸乙酯相浸膏进行了分离,通过1H-NMR、13C-NMR、EIMS等方法鉴定了10个化合物:Kalamycin(1),Medermycin(2),Menoxymycin-B(3),spatozoate(4),邻氨基苯甲酸(5),3-吲哚乙醇(6),1-乙酰基咔啉(7),对羟基苯基乙醇(8),吲哚-3-乙酸(9),吲哚-3-甲酸(10)。

Ten compounds including Kalamycin （1）, Medermycin （2）, Menoxymycin-B （3）, spatozoate （4）, anthranilic acid（5）, 3-indolylethanol（6）, 1-acetyl-[3-carbolin（7）, p-hydroxyphenylethyl alcohol（8）, indole-3-acetic acid（9）, and indol-3-carboxylic acid（10） were isolated from the ethyl acetate extract of a marine-derived Streptomyces sp. L211 by using normal phase silica gel, Sephadex LH-20 chromatography and preparative thin layer chromatography. Their structures were identified by EIMS. spectroscopic methods including 1H-NMR, 13C-NMR and