PD-1和TIM-3在胃癌病人免疫组织CD3^+T细胞中的表达分析

Increased expression of PD-1 and TIM-3 on CD3^+T cells from gastric cancer patients

目的探讨在胃癌病人外周血、癌旁组织和肿瘤组织中T细胞上PD-1和TIM-3的表达状况及意义。方法应用流式细胞术检测来自胃癌病人外周血、癌旁组织和肿瘤组织及正常供体外周血的CD3+T细胞,分析PD-1和TIM-3的表达水平。结果PD-1和TIM-3在胃癌病人T细胞上表达水平明显高于正常供体。相比胃癌病人外周血,肿瘤组织TIL的PD-1和TIM-3表达水平更高。另外,PD-1和TIM-3的表达水平与胃癌病人的年龄和肿瘤大小存在相关性。通过阻断PD-1和TIM-3信号通路可以增强TIL抗肿瘤细胞毒性。结论 PD-1和TIM-3可能与胃癌的恶变有关。单克隆抗体靶向阻断PD-1和TIM-3通路有希望成为胃癌免疫治疗的新方法。

To determine the expression of PD-1 and TIM-3 on T cells in tumor tissue,adjacent gastric mucosa and PBMC from patients with gastric cancer,flow cytometry was used to test the expression of PD-1 and TIM3 on CD3＋T cells from tumor tissue,adjacent gastric mucosa and PBMC,as well as from normal donor PBMC.The levels of PD-1 and TIM-3 expressed on T cells from gastric cancer patients were significantly higher than those of normal donors.The percentages of tumor infiltrating lymphocytes（TILs） expressing PD-1 and TIM-3 were significantly higher than that in PBMC of gastric cancer patients.Moreover,the expression of PD-1 and TIM-3 was associated with the age and tumor size of patients.TILs cytotoxicity against tumor was enhanced when blocking PD-1 and TIM-3 pathways.Therefore,our results indicate that PD-1 and TIM-3 may play a pivotal role in the progression of gastric cancer and blockade of PD-1 and TIM-3 pathways using monoclonal antibodies could be a promising immunotherapy for gastric cancer.