棘球蚴病患者血清中可溶性Tim3/Galectin9的变化

The change of soluble Tim3 and Galectin-9 in sera of patients with echinococcosis

目的研究棘球蚴病患者血清中可溶性Tim3(sTim3)和Galectin9及Th1(IFN-γ)和Th2(IL-4)细胞因子水平变化在棘球蚴病感染过程中的相关作用。方法通过酶联免疫吸附试验ELISA法检测sTim3/Galectin9的表达水平;采用CBA法检测棘球蚴病患者血清中Th1/Th2细胞因子的分泌水平。结果棘球蚴病患者血清中sTim3的水平升高[(310.927±80.750)pg/ml,P=0.001],Galectin9水平升高[(1.690±1.118)ng/ml,P=0.009],且sTim3和Galectin9表达呈正相关(r=0.29,P=0.04),Th2相关细胞因子IL-4的水平升高(P=0.000),而Th1相关细胞因子IFN-γ水平下降(P=0.000),sTim3与IFN-γ呈负相关关系(r=-0.46,P=0.001)。结论在棘球蚴病感染过程中sTim3和Galectin9水平明显增高,可能有助于棘球蚴发生发展,并在其感染过程中起一定作用。

To investigate the roles of sTim3 and Galectin9 as well as Th1 and Th2 cytokines（IFN-γ and IL-4）in echinococcosis,we analyzed sTim3/Galectin9 in sera of patients with echinococcosis by ELISA and measured Th1/Th2 cytokines level by cytometric bead array（CBA）.ELISA demonstrated that concentration of sTim3 in sera of echinococcosis patients was elevated [（310.927±80.750） pg/ml,P=0.001],and the level of Galectin9 was elevated simultaneously [（1.690±1.118） ng/ml,P=0.009],furthermore,the expressions of sTim3 and Galectin9 were positive correlated（r=0.29,P=0.04）;CBA show that the level of Th2 cytokine（IL-4） was elevated（r=0.29,P=0.04） but the level of Th1 cytokine（IFN-γ） was decreased（P=0.000）.In conclusion,the experiment shows that the levels of sTim3 and Galectin9 are elevated and the expression of Th1/Th2 cytokines is imbalance in echinococcosis,which may lead to immune imbalance,thus boost the echinococcosis immune escape.