小鼠不同途径感染真菌模型的建立及LD50的测定

Establishment of Mouse Model with Different Ways of Fungal Infection and the Determination of the LD50

目的：建立一种稳定的真菌感染的小鼠模型。方法选用健康6～8周龄的BALB/c雌性小鼠，体重16～18g，选择静脉和鼻饲给药，每天进行死亡情况观察，连续观察26～31d，计算小鼠的半数致死量（LD50）。结果经尾静脉注射白假丝酵母菌给药小鼠高剂量组（2×10^7）全部死亡，中剂量组（2×10^6）4只死亡，低剂量组（2×10^5）没有死亡，鼻饲给药组小鼠高中低剂量均无死亡，说明小鼠的死亡与剂量高低、给药途径有直接关联的小鼠出现死亡。结论在利用小鼠做真菌感染的实验时，尽可能选择鼻饲给药。

Objective To obtain a stable mouse model of fungal infection .Methods Female BALB/c mice,6 to 8-weeks-old,weighing 16 to 18g,were used for 50% lethal dose （ LD50） evaluation of Candida albicans , which were injected intravenously and intranasally .Mice were kept under observation for 31 days and deaths were recor-ded on a daily basis .Results Mice with tail vein injection of Candida albicans in high dose group （2 ×10^7 ） all died, four death in the medium dose group（2 ×10^6）,no death in low dose group（2 ×10^5）.Nasal feeding mice had no death in all groups ,saying that dose and routes of administration were directly related to the death of mice .Conclusion The intranasal route provided a simple ,non-invasive murine model for investigating fungal infection .