CIP2A和OPN在膀胱癌中的表达及其意义

Expression and correlation of CIP2A and OPN in bladder cancer

目的 探讨蛋白磷酸酶2A的癌性抑制因子（CIP2A）、骨桥蛋白（OPN）在膀胱癌中的表达及其在膀胱癌发生发展中的作用.方法 对2010年5月至2012年12月山东大学第二医院泌尿外科收治的膀胱癌患者,采用反转录PCR （RT-PCR）检测38例患者肿瘤组织和12例患者癌旁正常膀胱组织中CIP2A mRNA、OPN mRNA的表达;免疫组化染色法检测99例患者肿瘤组织和12例患者癌旁正常膀胱组织中CIP2A、OPN蛋白的表达.结果 RT-PCR显示38例膀胱癌患者中29例（76.32％）检测到CIP2A mRNA的表达,35例（92.11％）检测到OPN mRNA的表达,正常膀胱组织中二者均未有表达（均P＜0.05）.免疫组化结果显示99例膀胱癌患者中有63例（63.64％） CIP2A蛋白阳性,84例（84.85％）OPN蛋白阳性;而正常膀胱组织中未检测到CIP2A蛋白,2例OPN蛋白阳性（均P＜0.05）.99例膀胱癌患者中,CIP2A蛋白与OPN蛋白表达两者均阳性的58例（58.59％）,两者均阴性的13例,CIP2A阳性而OPN阴性8例,CIP2A阴性而OPN阳性20例（r=0.300,P＜0.05）.结论 CIP2A、OPN在膀胱癌组织中高表达,CIP2A、OPN可以作为反映膀胱癌生物学行为的有效指标,提示了两者作为诊断标志物联合应用的潜在价值.

Objective To explore the expressions of cancerous inhibitor of protein phosphatase 2A （CIP2A） and osteopontin （OPN）and evaluate their roles in bladder cancer.Methods RNA was isolated from 38 cases of patients with bladder cancer and 12 cases of normal bladder tissue by TRIzol method from May 2010 to December 2012.And reverse transcription （RT）-PCR was used to detect the expressions of CIP2A and OPN.The expression levels of CIP2A and OPN in 99 cases of patients with bladder cancer and 12 cases of normal tissue were detected by immunohistochemical staining.Results The positive expression rate of CIP2A mRNA and OPN mRNA were 76.32% （29/38） and 92.11% （35/38）in bladder cancer while there was no expression in normal tissue （both P 〈 0.05）.The positive rates of CIP2A and OPN protein were 63.64% （63/99） and 84.85% （84/99） in cases of bladder cancer tissues while CIP2A was not detected in normal tissues.The positive expression rate of OPN in normal tissues was 2/12 （both P 〈 0.05）.The CIP2A and OPN proteins were both expressed in 58/99 cases of bladder cancer tissues while neither of them was expressed in 13 cases.In 8 cases,CIP2A was expressed while OPN was not.In another 20 cases,OPN was expressed while CIP2A was not（r =0.300,P 〈 0.05）.Conclusions The expression levels of CIP2A and OPN in tissue of bladder cancer are higher than those of normal controls.And CIP2A and OPN may be used as indicators of biological behaviors and serve as new molecular diagnostic markers for bladder cancer.