丙泊酚对乏糖培养星形胶质细胞miR-181a及Bcl-2蛋白表达的影响

Effects of propofol on miR-181a and Bcl-2 expression in glucose deprivation cultured astrocytes

目的 探讨丙泊酚通过miR-181a调控Bcl-2蛋白表达对乏糖培养星形胶质细胞的影响.方法 原代培养小鼠星形胶质细胞,使用丙泊酚0、1、5、10、15、20 μmol/L处理12 h,更换乏糖培养24 h后,显微镜下计数星形胶质细胞存活率.观察反应性氧元件（ROS）产生和线粒体膜电位变化的影响;观察miR-181a及Bcl-2蛋白表达水平变化与丙泊酚对星形胶质细胞保护效应的关系.结果 丙泊酚及乏糖处理后,0、1、5、10、15、20 μmol/L丙泊酚处理组星形胶质细胞存活率分别为（0.51±0.03）％、（0.52±0.02）％、（0.52±0.02）％、（0.73±0.04）％、（0.31±0.02）％、（0.21±0.02）％,差异有统计学意义（F=118.62,P＜0.001）;其中10 μmol/L丙泊酚处理组高于0μmol/L丙泊酚处理组,15 μmol/L及20 μmol/L丙泊酚处理组低于0μmol/L丙泊酚处理组.10 μmol/L丙泊酚可以明显改善乏糖引起的氧化应激反应,增加线粒体膜电位稳定性.Bcl-2蛋白表达在10 μmol/L丙泊酚处理后明显升高,同时,miR-181a表达明显降低.结论 10 μmol/L丙泊酚对乏糖培养星形胶质细胞具有保护作用,与其抑制miR-181a并增强Bcl-2蛋白表达有关.

Objective To explore the propofol regulation of Bcl-2 protein expression through miR-181a in glucose deprivation （GD） cultured astrocytes.Methods Primary cultured murine astrocytes were treated with 0,1,5,10,15,20μmol/L propofol for 12 h and then cultured with GD medium for24 h.The cell survival rate was recorded with microscope.Reactive oxygen species （ROS） formation and mitochondrial membrane stabilization were observed.And the expression levels of miR-181a and Bcl-2 protein were recorded to analyze the protection effects of propofol on astrocytes.Results After the treatments of propofol and GD,the survival rates of O,1,5,10,15,20 μmol/L propofol groups were （0.51 ±0.03）%,（0.52±0.02）%,（0.52±0.02）%,（0.73 ±0.04）%,（0.31 ±0.02）% and （0.21 ±0.02）%.And there were statistical significance （F =118.62,P 〈0.001）.Compared with 0 μmol/L propofol group,the survival rate was much higher in 10μmol/L propofol group while much lower in 15 μmol/L and 20 μmol/Lpropofol groups.10 μmoL/L propofol could decrease ROS formation and stabilize mitochondrial membrane.And Bcl-2 protein expression was up-regulated while miR-181a expression inhibited by 10 μmol/L propofol.Conclusion The protection of 10 μmol/L propofol against GD stress in astrocytes is correlated with inhibiting miR-181 a and up-regulating Bcl-2 protein expression.