重症中暑肠淋巴激活血管内皮细胞炎性活性的研究

Inflammatory activity of endothelial cells activated by intestinal lymph in severe heat stroke

目的探讨重症中暑肠淋巴对血管内皮细胞炎性活性的影响。方法制备重症中暑大鼠模型,收集大鼠热暴露前(Pre-HS)、热暴露期间(During-HS)和重症中暑形成后(Post-HS)肠淋巴。分别采用5%肠淋巴孵育人脐静脉内皮细胞(HUVEC)ECV-340细胞3、6h,检测ECV-340细胞高迁移率族蛋白B1(HMGB1)、肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)和白介素6(IL-6)的分泌水平,以及细胞间黏附分子1(ICAM-1)mRNA表达和核转录因子κB(NF-κB)活性的变化。结果热暴露后大鼠肠淋巴可诱导ECV-340细胞HMGB1、TNF-α、IL-1β和IL-6分泌增加(P<0.05),且此诱导效应呈时间依赖性增强(P<0.05)。重症中暑肠淋巴诱导后,ECV-340细胞ICAM-1 mRNA表达和NF-κB活性显著上调且呈时间依赖性增强(P<0.05)。结论肠淋巴途径的肠源性毒性物质和血管内皮构成了重症中暑炎症反应发生的关键发病环节。

Objective To explore the effect of mesenteric lymph from rats suffering from heat stroke（SHS） on the inflammatory activity of vascular endothelial cells. Methods Rats were placed in a prewarmed incubator to reproduce heat stroke. Human umbilical vein endothelial cells（HUVECs ECV-340） were incubated in 5% mesenteric lymph collected pre-, during-, and post-HS for 3 and 6 h respectively in vitro. The levels of high mobility group protein B1（HMGB1）, tumor necrosis factor α（TNF-α）, interleukin 1β（IL-1β）, and IL-6 of ECV-340 cells were determined. The expression of intercellular adhesion molecule 1（ICAM-1）mRNA and NF-κB activity of ECV-340 were also assayed. Results The levels of HMGB1, TNF-α, IL-1β and IL-6 induced by SHS mesenteric lymph of rats significantly increased after heat treatment（P〈0.05）, and this effect was enhanced with prolongation of exposure time（P〈0.05）. The expression of ICAM-1 mRNA and NF-κB activity were significantly elevated after being activated by SHS mesenteric lymph（P〈0.05）. Conclusion HS mesenteric lymph probably activates the inflammatory responses of vascular endothelium, which is closely associated with the pathogenesis of SHS.