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沉默DJ-1基因表达对子宫内膜癌Ishikawa细胞增殖及凋亡的影响 被引量：5

Effects of DJ-1 gene silencing on proliferation and apoptosis of the endometrial carcinoma cell line Ishikawa
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摘要 目的 :探讨DJ-1基因沉默对子宫内膜癌Ishikawa细胞增殖及凋亡的影响。方法 :将针对DJ-1基因的特异性干扰质粒(pGPU6/GFP/neo-DJ-1-shRNA)转染子宫内膜癌Ishikawa细胞,然后采用MTT法检测pGPU6/GFP/neo-DJ-1-shRNA转染对Ishikawa细胞增殖的抑制作用,采用实时荧光定量PCR及蛋白质印迹法检测DJ-1mRNA及蛋白表达水平的变化,采用Annexin-Ⅴ/碘化丙啶(propidium iodide,PI)双染和FCM法检测细胞凋亡情况。结果 :pGPU6/GFP/neo-DJ-1-shRNA转染能够明显抑制Ishikawa细胞的体外增殖,并且该抑制作用具有浓度和时间依赖性(P<0.05)。pGPU6/GFP/neo-DJ-1-shRNA转染Ishikawa细胞后,DJ-1 mRNA及蛋白表达水平均明显低于各对照组(P<0.01),而细胞凋亡率明显高于对照组(P<0.01)。结论 :沉默DJ-1基因表达可抑制子宫内膜癌Ishikawa细胞增殖,促进细胞凋亡。 Objective： To investigate the effects of DJ-1 gene silencing on proliferation and apoptosis of endometrial cancer Ishikawa cells. Methods： After the specifi c interference plasmid pGPU6/GFP/neo-DJ-1-small hairpin RNA（shRNA） targeting DJ-1 gene was transfected into Ishikawa cells, the change of cell proliferation was detected by MTT method, the expressions of DJ-1 mRNA and protein were detected by real-time fl uorescence quantitative PCR and Western blotting, as well as the apoptosis of Ishikawa cells was detected by Annexin-Ⅴ/propidium iodide（PI） dual staining and fl ow cytometry, respectively. Results： The transfection of pGPU6/GFP/neo-DJ-1-shRNA signifi cantly inhibited the proliferation of Ishikawa cells in a dose- and time-dependent manner（P 〈 0.05）. The expressions of DJ-1 mRNA and protein in Ishikawa cells transfected with pGPU6/GFP/neo-DJ-1-shRNA were signifi cantly lower than those in the control groups（P 〈 0.01）. The apoptosis rate in pGPU6/GFP/neo-DJ-1-shRNA transfection group was signifi cantly higher than that of the control groups（P 〈 0.01）. Conclusion： DJ-1 gene silencing can inhibit the proliferation of endometrial cancer Ishikawa cells and promote the apoptosis.
作者 肖仲清 龙生根 朱其舟 严金金 梅彤 舒宽勇
机构地区 江西省妇幼保健院妇科肿瘤科
出处 《肿瘤》 CAS CSCD 北大核心 2014年第10期924-928,共5页 Tumor
关键词 子宫内膜肿瘤 RNA 小分子干扰 细胞增殖 细胞凋亡 DJ-1基因 Endometrial neoplasms RNA,small interfering Cell proliferation Apoptosis Gene,DJ-1
分类号 R737.33 [医药卫生—肿瘤]
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参考文献18

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二级参考文献13

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肿瘤
2014年 第10期

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