c-Myc和Bin1在人食管鳞状细胞癌组织中的表达及其临床意义

Expression and clinical significance of c-Myc and Bin1 in esophageal squamous cell cancer

目的:研究人食管鳞状细胞癌(esophageal squamous cell cancer,ESCC)组织及其癌旁组织中c-Myc和Bin1基因的表达情况,并分析其临床意义。方法:收集2013年4月至2014年3月在河北医科大学第四医院胸外科行肿瘤切除并经病理证实的ESCC患者肿瘤组织和相应癌旁组织标本各54例,使用RT-PCR和免疫组织化学法检测ESCC组织和癌旁组织中c-Myc和Bin1基因的mRNA和蛋白的表达。结果:与癌旁组织相比,ESCC组织中的c-Myc mRNA表达水平和蛋白阳性表达率均显著升高[(0.34±0.29)vs(0.17±0.16),P<0.001;55.56%vs 33.33%,P=0.033],Bin1 mRNA表达水平和蛋白阳性表达率均显著降低[(0.25±0.19)vs(0.33±0.20),P<0.001;57.41%vs 84.48%,P=0.007]。ESCC组织中c-Myc mRNA的表达与Bin1 mRNA的表达呈显著负相关关系(r=0.790,P<0.001),c-Myc蛋白表达与Bin1蛋白表达也呈显著负相关关系(P=0.019)。c-Myc和Bin1蛋白表达均与患者TNM分期、肿瘤侵犯深度、分化程度、淋巴结转移相关。结论:人ESCC患者肿瘤组织中c-Myc呈高表达,而Bin1呈低表达,两者表达呈负相关,均与ESCC进展和淋巴结转移有关联。

Objective： To investigate the expression of c-Myc and Bin1 in association with clinical characteristic in patients with esophageal squamous cell cancer（ ESCC）. Methods： Carcinoma and para-carcinoma tissue specimens were collected from 54 patients with esophageal squamous cell cancer who underwent esophagectomy in Fourth Hospital of Hebei Medical University between April,2013 and March,2014. Bin1 and c-Myc mRNA and protein levels were determined by RT-PCR and immunohistochemistry respectively. The associations between c-Myc and Bin1 protein levels and clinical features were analyzed. Results： Compared with para-carcinoma tissue specimens,carcinoma tissue specimens had significantly higher c-Myc mRNA abundance（ 0. 34 ± 0. 29 vs 0. 17 ± 0. 16,P〈 0. 001） and a significantly higher percentage of c-Myc protein-positive tests（ 55. 56% vs 33. 33%,P = 0. 033）. In contrast,Bin1 mRNA abundance was significantly decreased（ P 〈0. 001） in carcinoma tissue specimens（ 0. 25 ± 0. 19） as compared with para-carcinoma tissue specimens（ 0.33 ±0.20） and the percentage of Bin1 protein-positive tests was significantly lower in carcinoma specimens than in para-carcinoma specimens 57. 41% vs（ 84. 48%,P = 0. 007）. The abundance of c-Myc mRNA was negatively correlated with Bin1 mRNA abundance（ r =- 0. 790,P〈 0. 001） and c-Myc protein level was negatively correlated with Bin1 protein level（ P = 0. 019）. Both c-Myc and Bin1 proteins were associated with TNM stage,tumor invasion depth,tumor dif-ferentiation and lymph node metastasis. Conclusion： In ESCC tissue,the expression of c-Myc is up-regualted while the expression of Bin1 is down-regulated. Bin1 is negatively correlated with c-Myc at both mRNA and protein levels. The aberrations in both c-Myc and Bin1 is closely associated with tumor invasion and lymph node metastasis of ESCC.