LYRIC在人肝癌组织中的表达及其临床意义

LYRIC in hepatocellular carcinoma: Expression and potential prognostic significance

目的:检测LYRIC分子在肝癌和癌旁组织中的表达,探讨LYRIC分子与肝癌患者临床病理特征的关系及其对预后判断的指导意义。方法:收集2005年1月至2013年12月沈阳军区总医院和中国医科大学附属盛京医院的87例肝癌患者肝癌及其癌旁组织切除标本,采用Real-time PCR和免疫组织化学染色等方法从基因和蛋白水平检测肝癌患者的癌组织及相应的癌旁组织中LYRIC分子的表达,结合患者病理指标、病理分型和预后随访资料分析LYRIC分子表达的临床意义。结果:在基因水平检测发现,LYRIC基因在56例肝癌组织中表达高于癌旁组织,高表达率为64.7%(56/87);在蛋白水平检测发现,LYRIC蛋白在87例肝癌组织中均表达,且在肝癌组织中的表达显著高于癌旁组织(2.16±0.87 vs 1.63±0.88,P=0.000 1)。在肝癌组织中LYRIC分子的高、低表达可较好地预测肝癌患者的预后情况,LYRIC分子高表达患者预后较差(Log-rank10.236,P=0.001);多因素分析提示,LYRIC高表达(HR=2.19)、转移(HR=2.44)及肿瘤分期(HR=2.01)是影响肝癌总体生存期的独立危险因素,而与肿瘤直径及是否有病毒感染无关。结论:LYRIC分子在肝癌组织中高表达,且其表达水平与肝癌的临床分期、转移能力和临床预后密切相关,是肝癌潜在的预后判断指标。

Objective： To determine the expression profile of lysine-rich CEACAM1 coisolated（ LYRIC） in hepatocellular carcinoma（ HCC） and matched adjacent normal liver tissue. Methods： Matched biopsy specimens of the tumor proper and the corresponding adjacent normal mucosa were collected from 87 HCC patients who underwent surgical resection in China Medical University-Affiliated Shengjing Hospital and General Hospital of Shenyang Military Region. LYRIC mRNA and protein in these specimens were assessed by realtime PCR and immunohistochemical staining. The potential significance of LYRIC mRNA and protein level measurements in the prognosis of HCC were evaluated by multivariate analysis. Results： In 56（ 64. 7%） of the 87 matched specimens,LYRIC mRNA was significantly higher in HCC than in the adjacent mucosa. LYRIC protein was detected in both HCC and adjacent normal tissue in all 87 cases,but the level wassignificantly higher（ P〈 0. 001） in HCC（ 2. 16 ± 0. 87） than in adjacent normal tissue（ 1. 63 ± 0. 88）. Enhanced LYRIC expression was associated with poor prognosis（ Log-rank10. 236,P = 0. 001）. Metastasis（ HR = 2. 44）,elevated LYRIC expression（ HR = 2. 19） in HCC and tumor stage（ HR = 2. 01） were independent risk factors of overall survival.In contrast,overall survival was not associated with tumor diameters and virus infection. Conclusion： LYRIC expression is significantly up-regulated in HCC as compared with normal liver tissue. Both LYRIC mRNA and protein levels in HCC are closely associated with the molecular subtype and staging of the tumor,prognosis,suggesting that LYRIC may serve as a potential prognostic factor of liver cancer.