TIEG1对K562细胞凋亡及BCL-2/BAX、PTEN表达的影响

Effects of TIEG1 on K562 Cell Apoptosis and Expression of BCL-2/BAX,PTEN

本研究旨在观察TIEG1诱导K562细胞凋亡及BCL-2/BAX、PTEN表达的变化。用TIEG1 0、1、5、10和20ng/ml处理K562细胞,MTT法检测细胞生长抑制率。TIEG1 10.00 ng/ml处理K562细胞后流式细胞仪检测细胞凋亡,RT-PCR方法检测BCL-2/BAX及PTEN的表达。结果表明,TIEG1对K562细胞增殖具有抑制作用,且呈时间及剂量依赖性(r=0.52,P<0.05);处理细胞6、12、24和48 h后TIEG1 IC50值分别为48.19、18.72、9.5和3.85 ng/ml。TIEG1 10.00 ng/ml处理K562细胞0、6、12、24和48 h后凋亡率分别为(2.13±0.42)%、(7.79±0.71)%、(11.17±1.37)%、(24.66±0.29)%和(48.60±1.38)%,各组凋亡率相比较统计学差异具有显著性(P<0.05)。在TIEG1诱导K562细胞凋亡过程中,BCL-2表达逐渐减少(r=0.48,P<0.05),而BAX(r=0.69,P<0.05)及PTEN(r=0.57,P<0.05)表达逐渐增加。结论:TIEG1诱导K562细胞凋亡并抑制其增殖,且呈时间、剂量依赖性。在TIEG1诱导K562细胞凋亡的过程中,BCL-2/BAX及PTEN表达变化与K562细胞凋亡相关。

The aim of this study was to investigate the effect of TIEG1 on K562 cell apoptosis and expression of BCL-2/BAX,PTEN.The different concentration（0,1,5,10,20 ng/ml） of TIEG1 were used to treat K562 cells,the cell growth inhibition rate was detected by using MTT method.After treating K562 cells with 10.00 ng/ml TIEG1,the cell apoptosis was detected with flow cytometry.The RT-PCR was used to detected the expression levels of BCL-2/BAX and PTEN.The results showed that TIEG1 displays inhibitory effect on proliferation of K562 cells in time-and dose-dependent manner（r =0.52,P 〈 0.05） ; after K562 cells were treated for 6,12,24 and 48 h,the IC50 of TIEG1 were 48.19,18.72,9.5 and 3.85 ng/ml respectively.After treating K562 cells with 10.00 ng/ml TIEG1 for 0,6,12,24,48 h,the apoptosis rate were （2.13 ± 0.42） %,（7.79 ± 0.71） %,（11.17 ± 1.37） %,（24.66 ± 0.29） % and （48.60 ± 1.38） % respectively,and there was significant difference between groups（P 〈 0.05）.In process of K562 cell apoptosis,the expression level of BCL-2 gradually decreased（r =0.48,P 〈 0.05）,meanwhile the expression levels of BAX（r =0.69,P 〈 0.05） and PTEN（r =0.57,P 〈 0.05） gradually increased.It is concluded that TIEG1 can indue apoptosis of K562 cells and inhibit K562 cell proliferation in time-and dose-dependent manner.In apoptosis process of K562 cells induced by TIEG1,the expression changes of BCL-2/BAX and PTEN associate with the K562 cell apoptosis.