多发性骨髓瘤患者血浆HSP90的表达及其临床意义

Expression and Significance of HSP90 in Plasma of Patients with Multiple Myeloma

本研究检测多发性骨髓瘤(MM)患者外周血HSP90的表达情况,探讨其在MM发病中的作用及与疾病治疗、预后转归的关系。收集58例MM患者和20例健康志愿者的外周血,应用双抗体夹心ELISA法检测血浆HSP90的表达水平。结果表明,MM患者外周血HSP90的表达水平明显高于对照组[(325.205±75.942)vs(85.401±11.261)ng/ml](P<0.001)。HSP90表达水平与MM患者的性别、年龄和疾病分型无关(P>0.05),而与疾病国际分期系统(ISS)的分期、疗效、浆细胞百分比和球蛋白、免疫球蛋白、M蛋白、β2-微球蛋白和轻链水平明显相关(P<0.05)。结论:HSP90在MM患者外周血中的过度表达可能参与了MM发病和进展,检测外周血HSP90的表达水平可能对判断MM患者的病情、疗效和预后有一定临床指导意义。

This study was purposed to investigate the expression of heat shock protein 90 （HSP90） in peripheral blood plasma of patients with multipl myeloma（MM）,and to explore its possible role in the pathogenesis of MM,and its relationship with treatment,prognosis and the outcome of patients.The peripheral blood samples from 58 patients with MM and 20 healthy volunteers were collected.The plasma concentration of HSP90 in patients and healthy volunteers was measured by ELISA.The results showed that the concentration of HSP90 in peripheral blood of patients with MM was significantly higher than that in the healthy volunteers [（32.398 ± 3.674）vs （25.762 ± 2.916）ng/ml] （P 〈 0.001）.The concentration of HSP90 showed positively correlation with International Staging System（ISS） stage,therapeutic response,frequency of plasmocyte,globulin,immune globulin,M-protein,β2 micro-globulin,and light chain of MM patients （P 〈 0.05） ; while it showed little correlation with sex,age and type of MM patients （P 〉 0.05）.It is concluded that the HSP90 may be involved in the occurrence and development of MM.Detection of HSP90 in plasma would contribute to judge the clinical course,therapeutic efficacy and prognosis of MM patients.