中国江苏地区汉族RhD阴性个体RHCE基因型多样性研究

Polymorphisms of RHCE Gene among Serologic RhD Negative Donors of Han Population in Chinese Jiangsu Area

本研究探究中国江苏地区血清学RhD阴性献血者中,含有RHD基因的献血者和不含RHD基因献血者中的RHCE基因型。采用聚合酶链式反应-序列特异性引物(PCR-SSP)方法分析337例血清学RhD阴性无偿献血者的RHCE基因型;同时采用PCR-SSP扩增这些献血者RHD基因特异性位点。结果表明:337例血清学RhD阴性,其中RHD基因阳性献血者RHCE*C/C 20例,RHCE*C/c 62例,RHCE*c/c 24例,RHCE*C及RHCE*c基因频率分别为0.4811和0.5189;RHCE*E/E 0例,RHCE*E/e 25例,RHCE*e/e 81例,RHCE*E及RHCE*e基因频率分别为0.1179和0.8821。231例RHD基因阴性献血者中RHCE*C/C 3例,RHCE*C/c 34例,RHCE*c/c 194例,RHCE*C及RHCE*c基因频率分别为0.0866,0.9134;RHCE*E/E 0例;RHCE*E/e 15例;RHCE*e/e 216例;RHCE*E及RHCE*e基因频率分别为0.0325,0.9675。结论:在中国江苏地区RHD基因阴性人群中,RHCE基因主要以RHCE*ccee为主。中国江苏地区血清学RhD阴性的献血者中,RHCE基因型在含有RHD基因组和不含RHD基因组存在统计学差异。

This study was purposed to investigate the RHCE gene polymorphisms in Chinese Jiangsu Han blood donors with and without RHD gene among serologic RhD negative population.PCR with sequence-specific primers （PCR-SSP） was used to detect RHCE genotype in 337 serologic RhD negative,RHD gene positive donors.The RHD gene-specific polymorphisms were also determined by PCR-SSP in these donors.The results showed that among 337 serologic RhD negative,RHD gene positive donor 20 were RHCE ＊ C/C,62 RHCE ＊ C/c,24 RHCE ＊ c/c,25 RHCE ＊ E/e,and 81 RHCE ＊ e/e; the allele frequencies for RHCE ＊ C and RHCE ＊ c were 0.4811 and 0.5189,respectively; and for RHCE ＊ E and RHCE ＊ e 0.1179 and 0.8821.Among 231 RHD gene negative donors,3 were RHCE ＊ C/C,34 RHCE ＊ C/c,194 RHCE ＊ c/c,15 RHCE ＊ E/e,216 RHCE ＊ e/e; the allele frequencies for RHCE ＊ C and RHCE ＊ c were 0.0866 and 0.9134,respectively; and allele frequencies for RHCE ＊ E and RHCE ＊ e were 0.0325 and 0.9675,respectively.It is concluded that the most prevalent allele of RHCE gene was RHCE ＊ ccee in Chinese Han Jiangsu RHD gene negative population.There is statistical difference in RHCE genotype distribution among serologic RhD negative population with and without RHD gene.