胚胎早期乳腺原基体外培养对乳腺干细胞相关标记表达稳定性的影响

In Vitro Culture of Early Embryonic Mammary Anlagen Affects Expression Stabilities of Mammary Stem Cell-associated Markers

乳腺干细胞具有产生各类乳腺细胞的能力,其研究对于乳腺发育的认知、乳腺癌的治疗以及乳腺生物反应器的开发都具有重要意义。此外,伴随胎儿发育,胚胎期乳腺干细胞所经历的增殖、迁移、侵袭等过程是成体乳腺干细胞没有的,而这些经历与乳腺癌的发生过程非常相似。因此,针对胚胎乳腺干细胞的研究在医学上具有更重要的意义。Sca-1、CD29、CD49f和CD24都是经常被用于乳腺干细胞分离纯化的细胞表面标记。该研究分析了不同的体外处理条件和时间对于上述标记在小鼠乳腺原基中表达稳定性的影响,旨在为胚胎早期乳腺干细胞的分离、纯化以及鉴定奠定分子基础,也为胚胎乳腺发育和乳腺癌发生的相关研究提供支持。

Mammary stem cells（MaSCs） possess the ability to generate all differentiated cell types in the mammary gland. The researches of MaSCs are important and fundamental for understanding mammary gland development, developing therapy of breast cancer and studying mammary gland bioreactor. In particularly, during embryonic development, fetal MaSCs undergo proliferation, migration and invasion, which do not occur in the resting adult mammary gland, and do resemble the progression of breast cancer. Therefore, the researches of fetal MaSCs are meaningful, especially in the field of medicine. Sca-1, CD29, CD49 f and CD24 are specific cell surface markers that have ever been applied to purify MaSCs. Here, we analyzed the expression stabilities of these cell surface markers in mouse mammary anlagen, which were cultured in different conditions. Our research provides foundation to purify and identify MaSCs, and supports the studies of mammary gland development and breast cancer.