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瑞芬太尼痛觉过敏大鼠Delta阿片受体mRNA的表达水平 被引量：1

Expression of Delta-opioid Receptor in Rats with Remifentanil-induced Hyperalgesia
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摘要 目的：探讨切口痛-瑞芬太尼痛觉过敏大鼠背根神经节及海马Delta阿片受体mRNA表达水平的变化。方法：健康雄性SD大鼠32只，尾静脉置管后随机分为4组，瑞芬太尼组（R组）输注瑞芬太尼1μg·kg-1·min-1，切口痛组（I组）输注等体积生理盐水，甘氨酸组（G组）输注甘氨酸15μg·kg-1·min-1，对照组（C组）输注等体积生理盐水，最后一次痛阈测定后留取大鼠L4-L6背根神经节及海马标本，应用实时定量PCR方法测定Delta阿片受体mRNA的表达水平。结果：R组、I组、G组均发生痛觉过敏，且R组痛觉过敏的程度高于I组和G组；R组海马Delta阿片受体mRNA表达水平（4.359±1.031）显著高于C组、I组（2.373±1.014）和G组（2.411±0.326）（P＜0.05）；R组背根神经节Delta阿片受体mRNA表达水平（2.108±0.361）显著高于C组、I组（1.409±0.435）和G组（1.413±0.234）（P＜0.05）。结论：瑞芬太尼可在切口痛模型大鼠中引发痛觉过敏，其机制可能与背根神经节及海马Delta阿片受体mRNA表达上调有关。 Objective To investigate the changes in the expression of delta-opioid receptor in dorsal root ganglion and hippocampus in rats with incisional pain（IP）-remifentanil-induced hyperalgesia. Methods Thir-ty-two SD male rats（250-280 g） were randomly divided into 4 groups： group R, remifentanil 1 μg·kg-1·min-1 was infused intravenously;group I, the same volume of saline was infused intravenously;group G, glycine 15μg· kg-1·min-1 was infused intravenously; group C, the same volume of saline was infused intravenously. Each animal was sacrificed after the last behavioral measurement. The dorsal root ganglion and hippocampus were removed for the evaluation of the expression of delta-opioid receptor mRNA by quantitative RT-PCR. Results Remi-fentanil-induced hyperalgesia developed in group R, I and G. Comparing with group C, I （2.373 ± 1.014） and G （2.411 ± 0.326）, the expression of delta-opioid receptor mRNA in hippocampus was significantly up-regulated in group R （4.359 ± 1.031） （P〈0.05）. Comparing with group C, I （1.409 ± 0.435） and G （1.413 ± 0.234）, the expres-sion of delta-opioid receptor mRNA in dorsal root ganglion was significantly up-regulated in group R （2.108 ± 0.361） （P〈0.05）. Conclusion These data indicate that the increased expression of delta-opioid receptor mRNA in dorsal root ganglion and hippocampus is involved in remifentanil-induced-hyperalgesia.
作者 苑方 王海云 于泳浩 王国林
机构地区 天津医科大学总医院麻醉科
出处 《中国中西医结合外科杂志》 CAS 2014年第5期507-510,共4页 Chinese Journal of Surgery of Integrated Traditional and Western Medicine
基金 国家自然科学基金(30972847)
关键词 痛觉过敏 瑞芬太尼 Delta阿片受体 实时定量PCR 大鼠 Hyperalgesia remifentanil delta-opioid receptor qRT-PCR rats
分类号 Q95-33 [生物学—动物学] R971.1 [医药卫生—药品]
  • 相关文献

参考文献9

  • 1Silverman SM. Opioid induced hyperalgesia: clinical implications for the pain practitioner [J]. Pain Physician, 2009, 12(3): 679-684.
  • 2Joo DT. Mechanisms of opioid tolerance: emerging evidence and therapeutic implications [J]. Can J Anesth,2007, 54(12):969-976.
  • 3Cabafiero D, Campillo A, Celerier E, et al. Pronociceptive Effects of Remifentanil in a Mouse Model of Postsurgical Pain [J]. Anes- thesiology, 2009, 111 (6): 1334-1345.
  • 4Cabafiero D, Celerier E, Garcia-Nogales P, et al. The pro-nocicep- tive effects of remifentanil or surgical injury in mice are associated with a decrease in delta-opioid receptor mRNA levels: Prevention of the nociceptive response by on-site delivery of enkephalins [J]. Pain, 2009, 141(1-2):88-96.
  • 5Zhang X, Bao L, Guan JS. Role of delivery and trafficking of del- ta-opioid peptide receptors in opioid analgesia and tolerance [J]. Trends Pharmacol Sci, 2006, 27(6):324-329.
  • 6Joly V, Richebe P, Guignard B, et al. Remifentanil-induced postop- erative hyperalgesia and its prevention with small-dose ketamine [J]. Anesthesiology, 2005,103(1):147-155.
  • 7Celerier E, Gonzalez JR, Maldonado R, et al. Opioid-induced hy- peralgesia in a murine model of postoperative pain [J]. Anesthesiol- oaw. 2006. 104(3):546-555.
  • 8李依泽,王海云,郭东勇,杨卓,杜津,王国林.δ受体在瑞芬太尼诱发大鼠脊髓背角神经元NMDA受体微小兴奋性突触后膜电流中的作用[J].中华麻醉学杂志,2011,31(11):1344-1346. 被引量：3
  • 9Zhao M, Joo D T. Enhancement of spinal N-methyl-D-aspartate receptor function by remifentanil action at delta2opioid receptors as a mechanism for acute opioid2induced hyperalgesia or tolerance [J]. Anesthesiology, 2008, 109(2):308-317.

二级参考文献10

  • 1Nadal X,Banes JE,Kieffer BL,et al.Neuropathic pain is enhanced in delta-opioid receptor knockout mice.Eur J Neurosci,2006,23(3):830-834.
  • 2Waxman AR,Arout C,Caldwell M,et al.Acute and chronic fentanyl administration causes hyperalgesia independently of opioid receptor activity in mice.Neurosci Lett,2009,462(1):68-72.
  • 3Joly V,Richebe P,Guignard B,et al.Remifentanil-induced postoperative hyperalgesia and its prevention with small-dose ketamine.Anesthesiology,2005,103(1):147-155.
  • 4Bekhit MH.Opioid-induced hyperalgesia and tolerance.Am J Ther,2010,17(5):498-510.
  • 5Guntz E,Dumont H,Roussel C,et al.Effects of remifentanil on N-methyl-D-aspartate receptor:an electrophysiologic study in rat spinal cord.Anesthesiology,2005,102(6):1235-1241.
  • 6Lau CG,Zukin RS.NMDA receptor trafficking in synaptic plasticity and neuropsychiatric disorders.Nat Rev Neurosci,2007,8(6):413-426.
  • 7Hellier JL,Grosshans DR,Coultrap SJ,et al.NMDA receptor trafficking at recurrent synapses stabilizes the state of the CA3 network.J Neurophysiol,2007,98 (5):2818-2826.
  • 8Zhao M,Joo DT.Enhancement of spinal N-methyl-D-aspartate receptor function by remifentanil action at delta-opioid receptors as a mechanism for acute opioid-induced hyperalgesia or tolerance.Anesthesiology,2008,109(2):308-317.
  • 9Yamada H,Shimoyama N,Sora I,et al.Morphine can produce analgesia via spinal kappa opioid receptors in the absence of mu opioid receptors.Brain Res,2006,1083(1):61-69.
  • 10Scherrer G,Imamachi N,Cao YQ,et al.Dissociation of the opioid receptor mechanisms that control mechanical and heat pain.Cell,2009,137(6):1148-1159.

共引文献2

同被引文献40

  • 1姚鹏,邢准,孟凌新.不同剂量纳洛酮对吗啡术后镇痛病人血浆阿片样物质的影响[J].中国医科大学学报,2006,35(6):642-644. 被引量：3
  • 2Benyamin R, Trescot AM, Datta S, et al. Opioid complica- tions and side effects [ J ]. Pain Physician, 2008,11 ( 2 Sup- pl) :S105 - S120.
  • 3Chapman CR, Lipschitz DL, Angst MS, et al. Opioid pharma- cotherapy for chronic non-cancer pain in the United States: a research guideline for developing an evidence-base [ J ]. J Pain ,2010,11 ( 9 ) :807 - 829.
  • 4Manchikanti L, Vallejo R, Manchikanti KN, et al. Effective- ness of long-term opioid therapy for chronic non-cancer pain [ J]. Pain Physician,2011,14(2) :E133 - E156.
  • 5Lee M,Silverman SM, Hansen H,et al. A comprehensive re- view of opioid-induced hyperalgesia [ J ]. Pain Physician, 2011,14(2) :145 -161.
  • 6Aguado D, Abreu M, Benito J, et al. Effects of aaloxone on opioid-induced hyperalgesia and tolerance to remifentanil under sevoflurane anesthesia in rats [ J]. Anesthesiology, 2013,118(5) :1 160-1 169.
  • 7Yamamoto J, Kawamata T, Niiyama Y,et at. Down-regulation of mu opioid receptor expression within distinct subpopulations of dorsal root ganglion neurons in a routine model of bone cancer pain [ J ]. Neuroscience ,2008,151 (3) :843 - 853.
  • 8Cabafiero D, C616rier E, Garcia-Nogales P, et al. The pro-no- eieeptive effects of remifentanil or surgical injury in mice are associated with a decrease in delta-opioid receptor mRNA levels:prevention of the nocieeptive response by on-site de- livery of enkephalins [ J ]. Pain ,2009,141 ( 1 - 2 ) : 88 - 96.
  • 9Hoskin PJ,Hanks GW. Opioid agonist-antagonist drugs in acute and chronic pain states [ J ]. Drugs, 1991,41 (3) :326 - 344.
  • 10Lee SC, Yoburn BC. The effect of nimodipine on opioid an- tagonist-induced upregulation and supersensitivity [J]. Pharmacol Biochem Behav, 2000,66 ( 2 ) : 347 - 351.

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中国中西医结合外科杂志
2014年 第5期

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