被动吸烟对雄性小鼠生殖能力影响的研究

Influence of Passive Smoke on the Male Mouse Fertility

目的:研究香烟烟雾对雄性小鼠生育能力的影响。方法:在30 cm×30 cm×30 cm的密闭环境中,每日给7周龄C57/BL6J雄性小鼠吸2支烟,共6周。用ELISA法检测血清中尼古丁代谢产物可替宁的含量,检测吸烟小鼠的血氧分压、精子运动性、向前运动性以及子代小鼠的平均存活数、存活率等。结果:吸烟小鼠血清中可替宁的含量为16.07±3.52 ng/ml,小鼠在香烟烟雾处理时血氧含量降低,但在脱离烟雾环境后就可恢复到正常水平。香烟烟雾处理组小鼠精子的运动性,向前运动性升高,子代小鼠的平均存活数、存活率相对于未吸烟小鼠显著降低(P<0.01)。结论:成功建立了小鼠吸烟模型,小鼠吸烟模型相当于人每日吸20支烟的水平,本模型可用于研究香烟烟雾对小鼠生殖系统的影响。

Objective： To study the effects of cigarette smoke on fertility of male mice. Methods： The C57/BL6 J male mice were treated with cigarette smoke twice a day in a 30 cm×30 cm×30 cm air-tight cabinet, lasting for 6 weeks. The cotinine content in smoking mouse serum was detected by ELISA, along with the monitor of the oxygen content of smoking mouse, the sperm mobility and progressive motility, as well as the average birth number and the survival rate of the offspring of the smoking mice. Results： The cotinine content in smoking mouse serum was 16.07±3.52 ng/ml and was equivalent to the cotinine content of human who smoked 20 cigarettes a day. The pO2 was down when it＇s smoking, but it would recover after cigarette smoking. The sperm mobility and progressive motility of the smoking mice were both higher than those of no smoking mice. The average birth number and the survival rate of the offspring of the smoking mice were statistically significantly lower than those of no smoking mice. The mouse smoking model was considered to be credible. Conclusion： Our mouse smoking model was similar to the people who smoked 20 cigarettes a day, so this model could make contributions to the investigation of the smoking effect on reproductive system in smoking mice.