西洋参茎叶皂苷对脑梗死大鼠ET-1、VEGF及脑梗死面积的影响

Effect of panax quinquefolium saponin from stems and leaves on endothelin-1,vascular endothelial growth factor and infarct area in rats

目的探讨西洋参茎叶皂苷(PQS)对脑梗死大鼠内皮素-1(ET-1)、血管内皮细胞生长因子(VEGF)及梗死面积的影响。方法采用栓线法对大鼠右侧大脑中动脉进行栓塞并随机分成假手术组、模型对照组、尼莫地平组、PQS高、低剂量组,用药后检测各组神经功能缺损情况、大鼠脑组织中ET-1及VEGF含量、大鼠脑梗死面积,并对各组检测数据进行比较分析。结果 PQS高、低剂量组神经功能损伤恢复状况均比尼莫地平组效果明显(P<0.05);与模型对照组比较,尼莫地平组、PQS低、高剂量组、假手术组脑组织中ET-1含量呈现明显下降趋势(P<0.05),各组之间两两比较差异均有统计学意义(P<0.05);PQS高剂量组效果最好;PQS高剂量组VEGF含量均高于其余各组(P<0.05);PQS高剂量组脑梗死面积最小,效果明显(P<0.05),PQS随着剂量的增加,脑缺血区有明显好转,可见少量胶质细胞增生。结论 PQS对提高血管活性、改善脑梗死病情具有显著效果。

Objective To explore the effect of panax quinquefolium saponin （PQS）from stems and leaves on endothe-lin-1 （ET-1 ）,vascular endothelial growth factor （VEGF）and cerebral infarct area in rats.Methods Rats were ran-domly divided into sham operation group,model control group,nimodipine group,high doses of PQS group and low doses of PQS group,after the right cerebral arteries were bolted to induce embolization.The neurological deficit,ET-1 and VEGF levels,and infarct area in each group were detected and compared.Results The neurological function recovered better in high and low doses of PQS groups than in other groups （P〈0.05 ）.Compared with the model control group,the other groups had significantly declined ET-1 expressions （P〈0.05）,and there was statistical differ-ence between any two groups （P〈0.05）.The high doses of PQS group had the highest VEGF expression but the least infarct area compared with the other groups.Conclusion Panax quinquefolium saponin from stems and leaves has a significant effect to enhance vascular activity and thus relieve cerebral infarction.