多b值弥散加权成像对早期兔脊柱结核模型的评价

Multiple b values in diffusion-weighted imaging of rabbit lumbar vertebra tuberculosis model early appraisal

目的:成功构建兔脊柱结核动物模型,探讨多b值弥散加权成像对早期脊柱结核的应用价值。方法:外科手术成功建立脊柱结核动物模型并将其分为病变组(30只)、对照组(10只),术后4周、6周、8周、10周后分别对两组进行多b值弥散加权成像检查,观察两组椎体表观弥散系数(ADC值)并拟合ADC图,术后10周后行组织病理学检查。结果:1病变组ADC值与对照组ADC值存在统计学差异(P<0.05);2随着b值增加,ADC值逐渐减小,同一时期,ADClow值与ADChigh值之间无统计学差异(P>0.05),而ADC3b值、ADC7b值以及ADCperf值均有统计学差异(P<0.05);3第4周ADC3b值、ADC7b值以及ADCperf值分别与6周、8周、10周的ADC3b值、ADC7b值以及ADCperf值之间存在统计学差异(P<0.05)。结论:成功构建与人类脊柱结核病理变化相似的兔脊柱结核,MRI能够为脊柱结核早期诊断提供一定信息,其中MRI的多b值弥散加权成像扫描方法对脊柱结核早期诊断更为敏感。

Objective：To construct the animal model of rabbit spinal tuberculosis,and to discuss applications of multiple b values in diffusion-weighted imaging of spinal vertebra tuberculosis.Methods：Firstly,animal models of spinal tuberculosis were built successfully by surgery and divided into two groups.Then they were examined with imaging and pathology at 4 weeks,6 weeks,8 weeks and 10 weeks respectively.Results：①There was statistical difference in lesion group of ADC values between the lesion group and the normal group （P＜0.05）; ②With the increase of b value,ADC values decrease gradually.At one time point,there was no statistical difference between ADClow values and ADChigh values（P＞0.05）,but there was statistical difference among ADC3b values,ADC7b values and ADCperf values （P＜0.05）; ③At 4 weeks,ADC3b values,ADC7b values and ADCperf values were statistically different from those at 6 weeks,8 weeks and 10 weeks（P＜0.05）.Conclusion：MRI can provide early diagnosis of spinal tuberculosis with some information,and DWI in MRI scanning is more sensitive to early diagnosis of spinal tuberculosis.Pathology of rabbit model with spinal tuberculosis is similar to that of human.