摘要
目的探讨激光熔覆后钛表面多孔形貌对细胞黏附、增殖和分化的影响。方法在氩气保护环境中应用脉冲式掺钕钇铝石榴石晶体(Nd:YAG)对45μm钛粉颗粒在钛片表面进行激光熔覆,采用场发射扫描电镜(SEM)观察钛片表面微观形貌,能量弥散X射线谱(EDS)检测钛片表面成分;采用小鼠骨髓基质干细胞BMSCs体外培养评价喷砂酸蚀组(A组)和激光熔覆组(B组)的生物相容性。SEM观察BMSCs在钛表面的黏附状况,细胞计数试剂盒(CCK-8)检测法和碱性磷酸酶(AKP)活性检测评价钛片对BMSCs增殖和分化的影响。结果与A组比较,B组互相连通的多孔钛表面更有利于BMSCs的黏附,也有利于BMSCs细胞的增殖和分化。结论激光熔覆处理钛片表面可促进BMSCs黏附、增殖及分化,有望成为一种有效有前景的钛表面处理方法。
Objective This work focuses on the titanium surface with porous structure obtained by laser cladding and evaluation of cell adhesion, proliferation and differentiation. Methods The specimens were prepared by a pulsed Nd ： YAG laser cladding of titanium powder with a particle size of 45 μm on it in an argon atmosphere. The microstructure and chemical composition analyses were performed by sacnning electron microscopy （SEM） and ener- gy dispersion spectroscopy（EDS）. SEM, CCK-8 method and alkaline phosphatase activity assay were taken to eval- uate the effect of the adhesion, proliferation and differentiation of mice bone marrow stem cells. Results The titani- um surface microstructure of multilevel and interconnected holes was proved of good cell biocompatibility. Conclu- sion Laser cladding proves to be an efficient means of surface modification of dental implants and the modified surface has a significant effect on the adhesion, proliferation and differentiation of BMSCs.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第11期1566-1569,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省教育厅自然科学研究项目(编号:KJ2009A037Z)
安徽省科技厅年度重点科研项目(编号:12070403070)
安徽省学术和技术带头人科研活动经费资助(编号:2014H030)
关键词
纯钛
激光熔覆
骨髓基质干细胞
表面处理
pure titanium
laser cladding
bone marrow stem cells
surface modification
