不同病理类型结直肠息肉中5-LOX与PlGF的表达及意义

Expression of 5-lipoxygenase and placental growth factor in different pathological type of colorectal polyps

目的探讨5-脂氧合酶(5-LOX)、胎盘生长因子(PlGF)及血清超氧化物歧化酶(SOD)、丙二醛(MDA)在不同病理类型结直肠息肉组织中的表达及意义。方法采用免疫组化法检测30例增生性息肉、30例结直肠腺瘤(15例高级别上皮内瘤变、15例低级别上皮内瘤变)、30例结肠癌及15例正常结肠黏膜中5-LOX、PlGF的表达情况,并采用ELISA法检测外周血SOD和MDA活性。结果与正常结肠黏膜组织比较,5-LOX、PlGF在增生性息肉、低级别上皮内瘤变、高级别上皮内瘤变及结直肠癌中表达均显著增加,呈递增趋势(P<0.05);与增生性息肉比较,低级别上皮内瘤变、高级别上皮内瘤变及结直肠癌之间差异有统计学意义。血清SOD活性显著降低,呈递减趋势(P<0.05),而MDA活性显著增强,呈递增趋势(P<0.05)。结论结直肠腺瘤性息肉向腺癌恶性转化过程中5-LOX、PlGF表达水平增加,呈递增趋势,可能是早期分子事件。

Objective To investigate the expression levels of 5-1ipoxygenase （5-LOX） and placental growth factor （ PIGF ） and serum activity of superoxide dismutase （ SOD ） and methane dicarboxylic ahlehyde （ MDA ） in different pathological types of colorectal polyps. Methods 30 cases of hyperplastic polyps, 30 cases of colorectal adenomas （15 cases of high level intraepithelial neoplasia, 15 cases of low level intraepithelial neoplasia）, 30 cases of colon cancer and 15 cases of normal colonic mucosa were enrolled in this study. Expressions levels of 5-LOX and P1GF were analyzed by immunohistochemical method and serum activities SOD and MDA were evaluated by ELISA as- says. Results Expressions of 5-LOX and P1GF in hyperplastic polyp, different levels of intraepithelial neoplasia and colorectal cancer were significantly higher than those of normal colon mucosa tissue （ P 〈 O. 05 ）. Compared with hyperplastic polyp, expression differences in low level intraepithelial neoplasia, high level intraepithelial neo- plasia and colorectal cancer expression were statistically significant. Serum activity of SOD in low level intraepithe- lial neoplasia, high level intraepithelial neoplasia and colorectal cancer was significantly lower than that in normal colon mucosa tissue （P 〈 0. 05 ）, while the MDA activity was significantly increased （ P 〈 0. 05 ）. Conclusion Expressions of 5-LOX and PIGF are significantly enhanced at an increasing trend in the process of malignant trans-formation of colorectal adenomas, which may be an early molecular event.