人参皂苷Rg1对植入痴呆大鼠海马中BMSCs存活、分化的影响及其机制

Study on the effect and mechanism of ginsenoside Rg1 on survival and differentiation of implanted BMSCs in dementia model rats

目的:探讨骨髓间充质干细胞(BMSCs)植入痴呆大鼠海马中的存活、分化情况及人参皂苷Rg1对其的影响及其机制。方法:雄性SD大鼠45只,采用随机数字法分为双侧穹窿海马伞(FF)切断模型组(模型组):阿尔茨海默病(AD)模型;BMSCs移植治疗组(BMSCs组):在模型制作2周后,每只大鼠接受1×106个BMSCs治疗;间充质干细胞联合人参皂苷Rg1治疗组(联合治疗组):两者联合与BMSCs组同时进行。采用免疫组织化学法检测海马BrdU阳性细胞数,免疫组织化学双染法检测NSE、BrdU双染阳性细胞,RT-PCR技术检测海马神经生长因子(NGF)mRNA表达。结果:模型组在FF切断术后6周,联合治疗组BrdU阳性细胞数(34.2±5.4)较BMSCs组(10.3±4.3)多(P<0.05)。联合治疗组海马NGF mRNA表达水平(1.13±0.21)较BMSCs组(0.75±0.12)有所升高(P<0.05)。结论:人参皂苷Rg1能够促进植入AD模型大鼠海马中的BMSCs生存,其机制可能与人参皂苷Rg1能够提高AD模型大鼠海马组织NGF mRNA的表达有关。

Objective：To explore the effect and mechanism of ginsenoside Rg1 on survival and differentia-tion of implanted BMSCs in dementia model rats. MethodForty ifve male SD rats were randomly divided into the FF transected model group （model group：ambi-hippocampal ifmbria-fornix transected）, the bone marrow mesenchymal stem cells （BMSCs treatment group）：Two weeks after the model-made, every rat received trans-plantation of BMSCs （10 μL, 1＆#215;106 cells） into hippocampus on both sides by Hamilton microinjector with ste-reotaxis, combining group （ginsenoside Rg1 combining BMSCs treatment group） received both transplantation of BMSCs and peritoneal infusion of ginsenoside Rg1 （Two weeks after the model-made, every rat received perito-neal infusion of ginsenoside Rg1 （5 mg/kg）, one day a time, until a month）. Survival and migration of implanted BMSCs were observed by immunohistochemistry staining. Differentiation of BMSCs was observed using immu-nohistochemistry double staining. In order to explore the mechanism of ginsenoside Rg1 increased the number of implanted BMSCs, RT-PCR technique was used to detect the change of NGF mRNA expression in hippocampus. ResultSix weeks after the model-made, The number of BrdU positive cells in hippocampus of combining treat-ment group （34.2±5.4） was more than those of simple BMSCs treatment group （10.3±4.3）, difference was signiif-cant （P＆lt;0.05）. The level of NGFmRNA expression in hippocampus of combining treatment group （1.13±0.21） was higher than that of BMSCs treatment group （0.75±0.12）, difference was signiifcant. Conclusion：ginsenoside Rg1 is beneift to the survival of implanted BMSCs. Ginsenoside Rg1 may facilitate the survival of implanted BMSCs by up-regulating content of NGF mRNA in hippocampus.