同型半胱氨酸对神经小胶质细胞IL-1β、IL-6表达的影响

Effects of homocysteine on expression of IL-1β, IL-6 in gitter cells

目的:研究同型半胱氨酸(HCY)对神经小胶质细胞(bv-2细胞)白介素-1β(IL-1β)、白介素-6(IL-6)表达的影响。方法:在细胞培养液中加入HCY、半胱氨酸(DL-CY)、空白对照组及溶剂对照组干预后,用细胞计数试剂盒(CCK8法)观察各组细胞增殖活性,并用酶联免疫吸附测定(ELISA)法测定细胞培养上清液IL-1β、IL-6浓度,用实时荧光定量核酸扩增检测系统(QPCR)的方法评价IL-1β、IL-6 mRNA表达改变。结果:实验各组中细胞增殖活性差异均无统计学意义(P>0.01),而HCY组IL-1β、IL-6的mRNA表达及上清液中蛋白表达与其他各组相比均有显著增加(P<0.01)。结论:HCY上调神经小胶质细胞IL-1β、IL-6的表达。

Objective：To investigate the effect of homocysteine on the expression of interleukin-1β（IL-1β）, interleukin-6 （IL-6） in gitter cells. MethodThe BV-2 cells were cultured in the cell culture lfuid for 24 hours. HCY/DL-CY or the control solvent were added, and a cell counting kit （CCK8） was used to observe the proliferation activity. Enzyme linked immunosorbent assay （ELISA） was used to evaluate the concentration of IL-1βand IL-6 in the cell culture supernate. Well, mRNA levels of IL-1βand IL-6 were analysed by Real-time Quantitative PCR Detecting System （QPCR）. ResultThe proliferation activity in each group were not signiif-cantly different, and the mRNA expression and protein expression of IL-1βand IL-6 in the supernatant homo-cysteine group was signiifcantly changed. Conclusion：Homocysteine increases the expression of IL-1β, IL-6 in gitter cells.