裸鼹鼠外周血白细胞免疫相关因子mRNA表达水平的检测

Detection on Level of Immune-related Factors mRNA in Peripheral Blood Leucocyte of Naked Mole Rats＇

目的 检测生长期和成年期裸鼹鼠外周血白细胞免疫相关因子mRNA表达水平,以了解裸鼹鼠的正常免疫水平.方法 通过眼眶静脉丛采集6月龄和12月龄裸鼹鼠外周血,提取外周血白细胞mRNA,反转录成cDNA后,实时定量PCR检测各组裸鼹鼠外周血中白细胞IL-1、IL-2、IL-4、IL-6、IL-8、IL-12、TN F-α、IFN-γ的mRNA表达水平.结果 除了IFN-γ、IL-4各组间无显著差异,6月龄裸鼹鼠的IL-2、TNF-α显著高于12月龄裸鼹鼠,尤其是6月龄雄性裸鼹鼠的表达水平显著较高.而细胞因子IL-1,IL-6中,6月龄雄性裸鼹鼠的表达水平也显著高于12月龄裸鼹鼠.另外,对细胞因子IL-8、IL-12表达检测结果表明,12月龄雌性裸鼹鼠的IL-8表达水平最低,而12月龄雄性裸鼹鼠的IL-12表达最低.结论 生长期雄性裸鼹鼠的细胞免疫水平和体液免疫水平均高于同龄的雌性动物和成年动物.

Objective To study the normal immunity function level of naked mole rats in growth period and adulthood, detection on mRNA expression levels of immune-related factor was conducted in 6-month and 12-month,old naked mole rats＇ peripheral leukocytes. Methods The peripheral blood was collected from orbital venous plexus in 6-months and 12-months-old naked mole rat. Total mRNA was extracted from peripheral blood leukocytes, and reversed into cDNA. Then, mRNA expression levels of IL-1, IL-2, IL-4, IL-6, IL-8, IL-12, TNF-α, IFN-γ was tested by real-time quantitative PCR. Results Apart from IFN-γ, IL-4, which is no significant difference between the groups, the mRNA expression of IL-2, TNF-α of 6-month-old naked mole rats were significantly higher than those of 12-month-old, especially the 6-month-old male group. The expression of IL-1, IL,6 in 6-month-old male group was also significantly higher than those of the 12-month-old groups. In addition, 12-month- old female group had the lowest IL-8 level, and 12-month-old male naked mole rats had the lowest IL-12 level. Conclusion The male naked mole rats in growing period had the higher immune level than their even-aged female and adults, whether in humoral or cellular immunity.