缺氧诱导因子-1α、血管内皮生长因子和生存素在非小细胞肺癌中的表达及相关性研究

Expression and correlation of hypoxia induced factor-1 alpha,vascular endothelial growth factor and Survivin in non-small cell lung cancer

目的:探讨缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)和生存素(survivin)在非小细胞肺癌中的表达及相关性。方法:收集我院收治的病理确诊为非小细胞肺癌患者20例,并分别取其癌组织与癌旁组织。采用免疫组织化学染色法检测癌组织与癌旁组织中HIF-1α、VEGF和生存素的表达。结果:HIF-1α、VEGF和生存素在非小细胞肺癌癌组织中的表达均高于其癌旁组织(P<0.05);在癌组织中,HIF-1α与VEGF和生存素表达呈正相关,Spearman相关系数分别为0.519、0.643;而VEGF与生存素表达亦呈正相关,Spearman相关系数为0.643(P<0.05)。结论:在非小细胞肺癌癌组织中HIF-1α、VEGF和生存素表达明显高于癌旁组织;HIF-1α可以调节VEFG和生存素的表达,且VEGF和生存素在肿瘤发生、发展过程中可能有一定的协同作用。

Objective： To explore the expression and correlation of hypoxia induced factor-1α（HIF-1α）, vascular endothelial growth factor （VEGF） and Survivin in non-small cell lung cancer （NSCLC）. Methods： The NSCLC tissue samples were collected from 20 patients confirmed pathologically and treated in our hospital. And twenty corresponding para-cancerous lung tissue specimens were obtained as a control group. The expressions of HIF-1α, VEGF and Survivin in the NSCLC tissues were detected by immunohistochemistry technique. Results： The expressions of HIF-1α, VEGF and Survivin in the NSCLC tissues was remarkably higher than those in para-cancerous lung tissues （P 〈 0. 05）. There was a positive correlation among HIF-1α, VEGF and Survivin in the NSCLC tissues, with the spearman correlation coefficient being 0. 519, 0. 643 or O. 643 respectively （P 〈 0. 05 ）. Conclusions： HIF-1α, VEGF and Survivin present a higher expression in NSCLC tissues than those in para-cancerous lung tissues. HIF-1α can up-regulate the expression of VEGF and Survivin, and there may be a certain synergy between VEGF and Survivin in the process of tumor occurrence and development. Key words Non-small cell lung cancer Immunohistochemistry Hypoxia induced factor-1 alpha Vascular endothelial growth factor Survivin