血清miR-96、miR-182对肝细胞性肝癌的诊断价值及其比较分析

Diagnostic application and comparative analysis of serum miR-96 and miR-182 in patients with hepatocellular carcinoma

目的:分析miRNA-96、miRNA-182在肝细胞性肝癌(hepatocellular carcinoma,HCC)诊断方面潜在的应用价值。方法:收集HCC、肝硬化(liver cirrhosis,LC)、慢性乙型肝炎(chronic hepatitis B,CHB)及正常对照血清各60例。以逆转录定量PCR方法检测miR-96、miR-182在血清中的相对表达量,以受试者工作曲线(receiver operating characteristic curve,ROC)分析两者在HCC诊断中的应用价值并与血清甲胎蛋白(alpha-fetoprotein,AFP)检测相比较。结果:HCC患者血清miR-96、miR-182相对表达量明显高于LC、CHB及正常对照组(FmiR-96=104.06,FmiR-182=318.23,均P<0.001),血清miR-96、miR-182 ROC下面积分别为0.901 5和0.936 8;miR-96对HCC诊断灵敏度和特异性分别为83.3%、80.8%,miR-182对HCC诊断灵敏度和特异性分别为85.0%、85.0%。两者诊断HCC灵敏度均优于血清AFP测定。结论:血清miR-96和miR-182是潜在的HCC诊断标志物。

Objective： To explore the diagnostic value of miRNA-96 and miRNA-182 in patients with hepatocellular carcinoma（HCC）. Methods： Gene expression of miR-96 and miR-182 were examined in 60 serum samples from liver cirrhosis（LC）,chronic hepatitis B（CHB） and health volunteers by quantitative real-time reverse transcription polymerase chain reaction（RTqPCR）. The diagnostic efficiency of miR-96 and miR-182 on HCC were analyzed by receiver operating characteristic curve（ROC） and compared with alpha-fetoprotein（AFP）. Results： Expression levels of miR-96 and miR-182 in HCC sera were significant higher than those in other groups（FmiR-96=104.06,FmiR-182=318.23, P〈0.001）. The areas under ROC of miR-96 and miR-182 in sera were 0.901 5 and 0.936 8. MiR-96 had a sensitivity of 83.3% and a specificity of 80.8%, miR-182 had a sensitivity of 85.0% and a specificity of 85.0% in discriminating HCC from LC or CHB, respectively. The HCC diagnostic sensitivity of miR-96 and miR-182 were better than AFP in sera. Conclusion： The expression levels of miR-96 and miR-182 in sera may be considered potential novel biomarkers for the diagnosis of HCC.