竹节参皂苷类化合物HPLC指纹图谱研究

Studies on the HPLC Fingerprints of Saponins in Rhizoma Panax Japonicus

目的:建立竹节参药材皂苷类成分HPLC指纹图谱的研究方法,为竹节参药材的质量控制提供依据。方法:采用Hibar C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%的磷酸水溶液为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长203nm,柱温30℃;测定了7批市售竹节参药材的HPLC指纹图谱。结果:竹节参的指纹图谱共检出12个共有峰,通过对照品比对指认了其中的7个共有皂苷类成分,7批药材中有6批相似度均达到0.9以上。结论:该方法精密度、稳定性、重复性好,特征性及专属性强,可用于竹节参药材的质量控制。

Objective：To establish HPLC fingerprint method for quality control of saponins in Rhizoma Panax Japonicus. Methods：The HPLC method was conducted based on a Hibar C 18 （4.6 mm ×250mm,5μm） column, with acetonitrile-0.1% orthophosphoric acid as mobile phase by gradient elution. The detection wavelength was at 203 nm. The flow rate was 1.0 mL· min^-1 and the column temperature was 30℃. Seven samples from different sources were analyzed by HPLC. Results ：Twelve main marker peaks were selected in the standard fingerprint and 7 peaks of saponins were identified. The similarity of 6 samples was over 0.9. Conclusion ： The precision, repeatability and stability of the method were up to standard,and this method can be used for quality evaluation of Rhizoma Panax Japonicus.