响应曲面法优化蕨麻多糖的提取工艺

Optimization of extraction process of polysaccharides from Potentilla Anserina L. by response surface methodology

以藏药蕨麻为试材,优化蕨麻多糖的提取工艺。在单因素实验的基础上,利用响应曲面法考察提取时间、温度和液料比对蕨麻多糖提取得率的影响,优化提取工艺,得到最优提取工艺条件:提取温度72℃、液料比28mL/g、提取时间102 min。提取2次,验证得到蕨麻多糖提取得率可达21.28%,接近于模型预测理论值21.44%。

The single factor test and response surface methodology were adopted to optimize the extraction process of polysaccharides from Potentilla Anserina L. Three factors including extraction temperature,extraction time and the ratio of liquid to material were selected with the Box-Behnken design. Response surface methodology showed that the optimal conditions of polysaccharides extraction were： extraction temperature 72 ℃,extraction time 102 min,the ratio of liquid to material 28 mL /g and twice for extraction. Under these conditions,the yield of polysaccharides was up to 21. 28%,which was well matched with the predictive yield of 21. 44%.