摘要
以亮叶桦(Betula luminifera)4个优良无性系为材料,通过1/2 MS、MS、B5、MS?B5四种基本培养基和分段扦插的方法筛选亮叶桦微扦插的最佳培养基以及外植体的最适茎段部位。结果表明:亮叶桦株高、根数、根长性状在不同培养基间存在极显著或显著差异,多重比较表明株高和根数表现最佳培养基为MS?B5,根长性状表现最佳培养基为B5。以不同部位的茎段为外植体,4个无性系在MS?B5培养基中微扦插,其株高和根数性状存在极显著差异,叶宽生长存在显著差异,而根长和叶长无显著差异,以株高生长为选择指标,最佳外植体为中下部茎段,以根数为选择指标,中上部茎段作为外植体优于下部茎段。综合分析表明,以中部茎段为外植体,选用MS?B5培养基,可适用于亮叶桦不同无性系的微扦插快繁。
Four different clones of Betula luminifera were selected for experiment of microcutting propagation in four types of medium with differentstem segments. The results showed that the height, rooting numbers and root length of cuttings had significant differences among different mediums.Multiple comparisons showed that the optimal medium was MS旴5 for height growth and rooting numbers, and B5 for root length. Different stemsegment of four clones in MS旴5 had significant differences of height growth, rooting numbers, and leaf width, except root length and leaf length.The experiments indicated that the explants from lower stem segments had better height growth, while the upper stem segments had more rootingnumbers.
出处
《浙江林业科技》
北大核心
2014年第4期79-82,共4页
Journal of Zhejiang Forestry Science and Technology
基金
浙江省竹木育种重大专项"光皮桦优质高产新品种选育及推广示范"(2012C12908-8)
关键词
亮叶桦
微扦插
无性系
Betula luminifera
micro-cutting
clones
