摘要
真核细胞的膜蛋白质，许多哪个是在各种各样的生物过程的关键播放器，多于药目标的一半组成并且代表结构的研究的重要候选人。与他们的生理的意义相对照，真核细胞的膜蛋白质结构的仅仅很有限的数字在 recombinant 蛋白质的产生由于技术挑战被获得了。在这评论，我们为真核细胞的膜蛋白质检验主要 recombinant 表示系统并且比较他们的相对优点和劣势。我们也试图在真核细胞的膜蛋白质纯化和结晶化的前进总结最近的技术策略。
Eukaryotic membrane proteins, many of which are key players in various biological processes, constitute more than half of the drug targets and represent important candidates for structural studies. In contrast to their physiological significance, only very limited number of eukaryoUc membrane protein structures have been obtained due to the technical challenges in the genera- tion of recombinant proteins. In this review, we examine the major recombinant expression systems for eukaryotic membrane proteins and compare their relative advantages and disadvantages. We also attempted to summarize the recent technical strategies in the advancement of eukaryotic membrane protein purification and crystallization.
基金
ACKNOWLEDGEMENTS We apologize to colleagues whose work could not be cited due to the scope of this review. We would like to thank members in Yan laboratory for discussions. We thank Brendan Lehnert, Xinlei Sheng, Quanxiu Li, Dan Ma and Xinhui Zhou for critical reading. This work was supported by funds from the National Basic Research Program （973 Program） （No. 2011CB910501）, the National Natural Science Foundation of China （Grant Nos. 31321062-20131319400, 31125009, and 91017011 ）, and funds from Tsinghua-Peking Center for Life Sciences. The research of N.Y. was supported in part by an International Early Career Scientist grant from the Howard Hughes Medical Institute.
关键词
真核生物
重组蛋白
结构测定
表达系统
膜蛋白
蛋白质纯化
生物过程
药物靶标
eukaryotic membrane proteins,recombinant expression, structural biology, integralmembrane proteins （IMPs）, fluorescence detected sizeexclusion chromatography （FSEC）, protein purification and crystallization
