齐刺胆的下合穴对胆绞痛豚鼠β-EP和CCK-8影响的研究

Effect on Endorphin(β-EP) and Cholecystokinin-octopeptide(CCK-8) of Biliary Colic Guinea Pigs by Triple Needling the Lower Confluent Point

目的:观察齐刺胆的下合穴对实验性胆绞痛豚鼠血浆β-内啡肽(β-EP)和八肽胆囊收缩素(CCK-8)的影响以及镇痛效果,为临床治疗胆绞痛选择新的方法提供科学依据。方法:将60只成年雄性豚鼠随机分为正常组(10只)、模型组(10只)、药物组(20只)和齐刺组(20只)。正常组不作任何处置正常饲养,模型组、药物组、齐刺组均造模。造模成功后,药物组予双氯芬酸钠盐酸利多卡因注射液腹腔注射;齐刺组取双侧阳陵泉穴进行齐刺,每日1次,连续治疗5天。治疗结束后15 min内处死各组豚鼠,腹主动脉采取新鲜血液,匀浆、离心后分离血浆,采用放射免疫法测定β-EP和CCK-8的含量。结果:模型组与正常组比较,血浆中β-EP和CCK-8含量显著升高(P<0.01);药物组和齐刺组血浆中β-EP和CCK-8含量均比模型组明显降低,有统计学意义(P<0.01);药物组、齐刺组比较无明显差异,无统计学意义(P>0.05)。结论:齐刺法刺激阳陵泉穴对实验性胆绞痛豚鼠有镇痛作用,可能与改善豚鼠血浆β-EP和CCK-8的表达水平有关。

Objective：To observe the effect of analgesia as well as the influence on endorphin （β-EP） and cholecystokinin-octopeptide （ CCK-8） in the plasma of the experimental biliary colic guinea, which provides a scientific basis for choosing a new way to treat biliary colic.Methods：60 adult male guinea pigs were divided into 4 groups： the normal group （ 10 guinea pigs ） , the model group （ 10 guinea pigs ） , the drug group （ 20 guinea pigs） and the triple needling group （20 guinea pigs）.The normal group was fed normally without any processing,but other groups made models.In the drug group, give a intraperitoneal injection with compound di-clofenac sodium and lidocaine hydrochloride injection after making models successfully; in the triple needling group, triple needled bilateral GB34 and needled once a day continuously treating for 5 days.At the end of the treatment, sacrifice the guinea pigs in each group, and fresh blood,homogenate were taken for the examination of theβ-EP and CCK-8 contents by radiation therapy.Results：Comparison between the model group and the normal group, the contents ofβ-EP and CCK-8 in the plasma obviously increased （ P＆lt;0.01）; compared with the model group, the contents of β-EP and CCK-8 in plasma both in drug group and triple needling group obviously decreased, which was statistically significant （ P＆lt;0.01）; no difference could be found be-tween the drug group and the triple needling group, which had no statistical significance （P＆gt;0.05）.Conclu-sion：Triple puncture at GB34 has an analgesic effect on experimental biliary colic guineas,and it is probably re-lated to the improvement of endorphin （β-EP） and cholecystokinin-octopeptide （CCK-8）’s expression levels in the plasma.