TYMS基因在64例肝癌患者中的表达

Expression of thymidylate synthetase gene in 64 primary hepatic cancer patients

目的分析胸苷酸合成酶（Thymidylatesynthetase，TYMS）基因在原发性肝癌中的表达及其临床意义。方法应用荧光定量PCR技术，对64例原发性肝癌TYMS基因进行扩增，免疫组化对照。结果在64例肝癌中有28例表达TYMS，占43．8％，拷贝数中位数M=3．55×10^4copies／mL，TYMS基因拷贝数对数（Logcopies）与免疫组化分值有相关性，r=0．97，其中高拷贝（〉1．0×10^4）表达有7例（10．9％），中拷贝（（1．0～10）×10^4）表达有10例（15．6％），低拷贝（〈10×10^3）表达有11例（17．2％），TYMS的表达与肿瘤大小、生存期（术后1年）、是否癌旁组织差异有统计学意义（P〈0．05），而与病理类型、AFP水平、HBV—DNA差异无统计学意义（P〉0．05）。结论荧光定量PCR技术可替代免疫组化方法检测TYMS基因的表达，TYMS基因在原发性肝癌有表达，对肿瘤生长和转移中有促进作用，可作为原发性肝癌的靶向治疗证据。

Objective To analye the expression of thymidylate synthetase（ TYMS ） gene in primary hepatic cancer cases. Methods Fluorescence quantitative PCR（FQ-PCR）was used to detect TYMS in 64 hepatocellular carcinoma cases and the results were ascertained with immunohistochemieal method . Results TYMSs was expressed in 64 primary liver cancer eases, occupied 43.8%, and the median copy number was M=3.55×10^4 copies/ml, being correlated with immunohistochemical score in TYMS gene copy number （ Log copies, r = 0.97） .High expression was noticed in seven cases （〉1.0× 10^5,accounted for 10.9%, moderate expression was noticed in 10 cases（（1.0-10） × 10^4 ） accounted for 15.6% and low expression （〈10× 10^3） l 1 eases （17.2%） . The expression of thymidylate synthetase in primary hepatic cancer cases revealed significant differences in tumor size, survival （ 1 year after operation）and paracaneerous tissue （P 〈0.05） .But there were no significant differences were found in pathological type, AFP level and HBV-DNA in the cases（P 〉0.05） .Conclusion FQ-PCR technology would take the place of immunohistoehemieal method for detecting TYMS gene in primary liver cancer patient. TYMS gene plays a part in promoting growth and metastasis of hepatic carcinoma it and might be taken as evidence of targeted therapy of primary liver cancer.