注射用三磷酸腺苷辅酶胰岛素对心肌缺血再灌注损伤所致心肌梗死的保护作用研究

Protective effects of energy mixture agent on myocardial infarction induced by myocardial ischemia-reperfusion in rats

目的研究注射用三磷酸腺苷辅酶胰岛素(能量合剂)对心肌缺血再灌注损伤所导致的心肌梗死大鼠的保护作用。方法结扎左冠状动脉前降支60 min后进行再灌注,与正常Ⅱ导联心电图(ECG-Ⅱ)比较,冠脉结扎后10 min,ECG-Ⅱ的ST段抬高0.2 mV以上者作为缺血成功;再灌注后10 min,ECG-Ⅱ中抬高的ST段下降30%以上者作为再灌注成功。造模成功后随机分为模型组,能量合剂组(3.6 mg·kg-1·d-1);另设8只大鼠作为假手术组。给药30 d后测定彩色多普勒超声心动图、血流动力学、心肌梗死范围;ELISA法检测血清丙二醛(MDA)、超氧化物歧化酶(SOD)、肌酸激酶同工酶(CK-MB)、肌酸脱氢酶(LDH)及心肌肌钙蛋白(cTnT)的表达。结果能量合剂可以改善心肌梗死大鼠的左心室功能,明显提高左心室的射血分数、降低左室舒张末压,减少心肌梗死范围,表明能量合剂能清除氧自由基,减少脂质过氧化产物的生成,从而起到保护损伤心肌的作用。结论能量合剂对心肌缺血再灌注损伤所导致的心肌梗死具有一定的保护作用。

Objective To investigate the therapeutic effects of energy mixture agent on myocardial infarction induced by myocardial ischemia-reperfusion in rats. Methods The acute myocardial infarction rat model was induced by ligation of the left anterior descending coronary artery for 60 min followed by reperfusion. The rats in which ST segment of Ⅱ lead electrocardiogram （ECG） was 0.2 mV more than the normal were as myocardial ischemia; The increased ST segment of Ⅱ lead ECG was reduced by 30% were as myocardial schemia-reperfusion. The model rats were divided into three groups randomly： model, energy mixture agent 3.6 mg·kg^-1·d^-1, and Sham group. The color Doppler echocardiography, cardiac hemodynamics, and range of myocardial infarction were measured; The blood levels of MDA, SOD, LDH, CK-MB, and cTNT were detected by ELISA after dosing for 30 d. Results Energy mixture agent could improve the left ventricular function in myocardial infarction rats, improve significantly the left ventricular ejection fraction, and reduce the left ventricular end-diastolic pressure and range of myocardial infarction, which suggested that energy mixture agent can scavenge the oxygen-free radicals and reduce the lipid peroxidative product-damaged heart tissue. Conclusion Energy mixture agant has significant protective effects on myocardial infarction induced by myocardial ischemia-reperfusion in rats.