自噬基因Atg5和MAPLC3在急性白血病中的表达及其意义

Expression and clinical significance of autophagy associated gene Atg5 and MAPLC3 in bone marrow mononuclear cells in patients with acute leukemia

目的探讨急性白血病(acute leukemia,AL)骨髓单个核细胞(bone marrow mononuclear cells,BMMNC)中自噬基因Atg5和MAPLC3的表达及其临床意义。方法采用荧光定量逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测150例AL患者和30例正常人BMMNC中Atg5和MAPLC3 mRNA的表达水平,对检测结果进行统计学分析。结果与对照组相比,急性髓系白血病(acute myelogenous leukemia,AML)各危险度组的Atg5和MAPLC3 mRNA表达水平均明显降低,且差异均有统计学意义(P均<0.01),从低危组、中危组至高危组,其Atg5和MAPLC3 mRNA表达水平逐步降低,组间两两比较,差异均有统计学意义(P均<0.01);与对照组相比,急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)各危险度组Atg5和MAPLC3 mRNA的表达水平均明显增高,差异均有统计学意义(P均<0.01),高危组患者的Atg5和MAPLC3mRNA表达水平明显低于标危组,差异均有统计学意义(P均<0.01);对照组和AL患者BMMNC中Atg5 mRNA水平与MAPLC3均呈显著正相关(P均<0.01)。结论自噬基因Atg5和MAPLC3 mRNA在AL中均有表达,且其表达水平呈正相关。AML患者的自噬活性明显降低,而ALL患者的自噬活性明显增高,随着危险度的增高,两种类型白血病自噬活性均星明显下降趋势。

Objective To investigate the expression and clinical significance of autophagy associated gene Atg5 and MAPLC3 in bone marrow mononuclear cells （BMMNC） in patients with acute leukemia（AL）. Methods Fluorescent quantitative reverse transcription-polymerase chain reaction （RT-PCR） was employed to detect Atg5 and MAPLC3 mRNA expression levels in BMMNC of 150 cases AL patients and 30 cases of healthy normals. The results were analyzed statistically. Results Compared with the control group, the Atg5 and MAPLC3 mRNA levels in all the risk groups of acute myelogenous leukemia （AML） were significantly reduced, and the differences all had statistical significance （Pall〈 0.01 ）. From the low-risk group , risk group to the high-risk group, the Atg5 and MAPLC3 mRNA levels gradually decreased, and the differences between each two groups all had statistical significance （Pall〈 0.01 ） ; Compared with the control group, the Atg5 and MAPLC3 mRNA levels in all the risk groups of acute lymphoblastie leukemia （ALL） were significantly increased, and the differences all had statistical significance （Pall〈0.01）. Atg5 and MAPLC3 mRNA levels in patients with high-risk group was significantly lower than that of standard risk group, the differences all had statistical significance （Pall〈 0.01 ） ; The Atg5 mRNA levels was positively related to the MAPLC3 levels in BMMNC of the control group and AL patients （Pall〈 0.01 ）. Conclusion The autophagy associated gene Atg5 and MAPLC3 are all expressed, and show positive correlation in AL patients. The autophagie activity in patients with AML is significantly reduced, while in patients with ALL is significantly increased. With increasing risk of both types of leukemia autophagic activity show a significantly decreased trend.