姜黄素预处理对大鼠肠缺血再灌注时肥大细胞活化的影响

Effect of curcumin preconditioning on activation of mast cells during intestinal ischemia-reperfusion in rats

目的 评价姜黄素预处理对大鼠肠缺血再灌注时肥大细胞活化的影响.方法 清洁级雌性SD大鼠24只,体重180～220 g,采用随机数字表法分为3组（n=8）：假手术组（S组）、肠缺血再灌注组（I/R组）和姜黄素预处理组（Cur组）.采用夹闭肠系膜上动脉75 min后恢复灌注的方法制备大鼠肠缺血再灌注损伤模型.于模型制备前5d,Cur组每天胃饲姜黄素200 mg/kg（采用生理盐水稀释）,S组和I/R组胃饲等容量生理盐水.于再灌注4h时放血处死大鼠,取肠组织标本,光镜下观察病理学结果并行Chiu评分,采用Western blot法检测类胰蛋白酶的表达,采用比色法检测β-已糖苷酶含量.结果 与S组比较,I/R组和Cur组大鼠肠组织Chiu评分和β-已糖苷酶含量升高,类胰蛋白酶表达上调（P＜ 0.05）;与I/R组比较,Cur组大鼠肠组织Chiu评分和β-已糖苷酶含量降低,类胰蛋白酶表达下调（P＜0.05）.结论 姜黄素预处理减轻大鼠肠缺血再灌注损伤可能与其抑制肠组织肥大细胞活化有关.

Objective To evaluate the effect of curcumin preconditioning on the activation of mast cells during intestinal ischemia/reperfusion （I/R） in rats.Methods Twenty-four female Sprague-Dawley rats,weighing 180-220 g,were randomly divided into 3 groups （n =8 each） using a random number table：sham operation group （S group）,intestinal I/R group （I/R group）,and curcumin preconditioning group （Cur group）.Intestinal I/R was produced by occlusion of superior mesenteric artery （SMA） for 75 min followed by reperfusion.At 5 days before I/ R,curcumin 200 mg/kg （in 20 mg/ml normal saline） was given through a gastric tube in Cur group,while the equal volume of normal saline was given instead in S and I/R groups.All the rats were sacrificed at 4 h of reperfusion,and the intestinal specimens were obtained for microscopic examination and for determination of tryptase expression and β-hexosaminidase content.Intestinal damage was assessed and scored according to Chiu.Results Compared with S group,Chiu＇s score and β-hexosaminidase content were significantly increased,and the expression of tryptase was up-regulated in I/R and Cur groups.Compared with I/R group,Chiu＇ s score and β-hexosaminidase content were significantly decreased,and the expression of tryptase was down-regulated in Cur group.Conclusion The mechanism by which curcumin preconditioning attenuates intestinal I/R injury is related to inhibited activation of mast cells of rats.